ArticleCell stem cell2016
Complete Meiosis from Embryonic Stem Cell-Derived Germ Cells In Vitro.
Article in Cell stem cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 192 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
192 citing papers in PubMed, 1 synthesis or guideline pooled it, 405 citations in OpenAlex.
- Update on fertility preservation from the Barcelona International Society for Fertility Preservation-ESHRE-ASRM 2015 expert meeting: indications, results and future perspectives.Human reproduction (Oxford, England) · 2017Guideline
- Molecular genetics of meiotic initiation in mammals.Biology of reproduction · 2026Article
- Endocrinology at a Miniature Level: Pluripotent Stem-Cell-Derived Organoid Models of Hypothalamus-Pituitary Axes.Biomolecules · 2026Review
- Considerations for the future of in vitro gametogenesis in fertility care.Nature biotechnology · 2026Article
- Reconstitution of spermatogenesis and continuous generation of functional haploid germ cells in mouse testicular organoids.Nature communications · 2026Article
- Establishment and optimization of the two-step induction system for generating primordial germ cell-like cells from chicken embryonic stem cells.FEBS open bio · 2026Article
- Advances in porcine stem cell research and their applications in agriculture.Frontiers in cell and developmental biology · 2026Review
- Stem Cells and Wildlife Conservation.Advances in experimental medicine and biology · 2026Review
- A simplified two-dimensional culture system supports meiotic progression during mouse spermatogenesis in vitro.PloS one · 2026Article
- Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular Organoids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- RAD21L1 Is Sufficient and Effective for Reprogramming Human Sertoli Cells to Phenotypic Spermatogonial Stem Cells Through DNA Methylation and Essential for Male Fertility.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A primordial germ cell-like-cell platform enables CRISPRi screen for epigenetic fertility modifiers.EMBO reports · 2025Article
- Single-cell analysis reveals cellular heterogeneity and molecular features during postnatal pig testis development.BMC genomics · 2025Article
- Stem cell-derived gametes: what to expect when expecting their clinical introduction.Human reproduction (Oxford, England) · 2025Review
- Initiation of meiosis from human iPSCs under defined conditions through identification of regulatory factors.Science advances · 2025Article
- Transcriptome-Wide Sequencing Identifies Non-Coding RNAs and Their Competing Endogenous RNA Networks During the Stages of pPGCLCs-Induced Differentiation.Stem cell reviews and reports · 2025Article
- Artificial Gametogenesis and In Vitro Spermatogenesis: Emerging Strategies for the Treatment of Male Infertility.International journal of molecular sciences · 2025Review
- Ethical, Legal and Social Issues in Utilizing In Vitro Gametogenesis (IVG) and Stem Cell-Based Embryo Models (SCBEMs) for Human Reproduction in Singapore.Health care analysis : HCA : journal of health philosophy and policy · 2025Article
- Application of induced pluripotent stem cells in the conservation of endangered animals.Stem cell research & therapy · 2025Review
- ImprovingJournal of biomedical research · 2025Article
132 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Erratum issued
Authors and funding
15 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vitro generation of functional gametes is a promising approach for treating infertility, although faithful replication of meiosis has proven to be a substantial obstacle to deriving haploid gamete cells in culture. Here we report complete in vitro meiosis from embryonic stem cell (ESC)-derived primordial germ cells (PGCLCs). Co-culture of PGCLCs with neonatal testicular somatic cells and sequential exposure to morphogens and sex hormones reproduced key hallmarks of meiosis, including erasure of genetic imprinting, chromosomal synapsis and recombination, and correct nuclear DNA and chromosomal content in the resulting haploid cells. Intracytoplasmic injection of the resulting spermatid-like cells into oocytes produced viable and fertile offspring, showing that this robust stepwise approach can functionally recapitulate male gametogenesis in vitro. These findings provide a platform for investigating meiotic mechanisms and the potential generation of human haploid spermatids in vitro.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.