Evidence map›Paper›PMID 26921944›Full record

ArticleMethods in enzymology2016

Dual-Color Luciferase Complementation for Chemokine Receptor Signaling.

Kathryn E Luker, Gary D Luker

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Kathryn E LukerDepartment of Radiology, Center for Molecular Imaging, University of Michigan, Ann Arbor, Michigan, USA. Electronic address: kluker@umich.edu.
Gary D LukerDepartment of Radiology, Center for Molecular Imaging, University of Michigan, Ann Arbor, Michigan, USA; Department of Biomedical Engineering, University of Michigan, Ann Arbor, Michigan, USA; Department of Microbiology and Immunology, University of Michigan, Ann Arbor, Michigan, USA.
University of Michigan–Ann Arbor · US

Funding

Systems Bioengineering of Cancer Cell MigrationR01CA196018 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LINDERMAN, JENNIFER JEAN, LUKER, GARY D · 2015 to 2019
$2.2M
Modeling Therapy of Disseminated Cancer Cells in Bone Marrow (PQ 17)R01CA170198 · NCI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LUKER, GARY D, TAKAYAMA, SHUICHI · 2012 to 2015
$1.4M
NCI NIH HHS R01 CA170198NCI NIH HHS R01CA170198NCI NIH HHS R01 CA196018NCI NIH HHS R01CA196018
6 · The paper itself

Abstract

Chemokine receptors may share common ligands, setting up potential competition for ligand binding, and association of activated receptors with downstream signaling molecules such as β-arrestin. Determining the "winner" of competition for shared effector molecules is essential for understanding integrated functions of chemokine receptor signaling in normal physiology, disease, and response to therapy. We describe a dual-color click beetle luciferase complementation assay for cell-based analysis of interactions of two different chemokine receptors, CXCR4 and ACKR3, with the intracellular scaffolding protein β-arrestin 2. This assay provides real-time quantification of receptor activation and signaling in response to chemokine CXCL12. More broadly, this general imaging strategy can be applied to quantify interactions of any set of two proteins that interact with a common binding partner.

Indexed as

beta-Arrestin 2Chemokine CXCL12Genetic Complementation TestLuciferasesLuciferases, FireflyLuminescent MeasurementsMolecular BiologyReceptors, ChemokineReceptors, CXCRReceptors, CXCR4Recombinant Fusion ProteinsSignal TransductionACKR3 protein, humanbeta-Arrestin 2Chemokine CXCL12CXCR4 protein, humanLuciferasesLuciferases, FireflyReceptors, ChemokineReceptors, CXCRReceptors, CXCR4Recombinant Fusion ProteinsBioluminescenceMolecular imagingOptical imagingProtein fragment complementationSplit luciferase

Identifiers

PMID26921944
PMCPMC5245831
OpenAlexW2218543388

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.