Evidence map›Paper›PMID 26921240›Full record

ArticleMarine biotechnology (New York, N.Y.)2016

Developmental Transcriptome Analysis and Identification of Genes Involved in Larval Metamorphosis of the Razor Clam, Sinonovacula constricta.

Donghong Niu, Fei Wang, Shumei Xie, Fanyue Sun, Ze Wang, Maoxiao Peng, Jiale Li

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

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  7. Dynamic response in the larval geoduck (Ecology and evolution · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Donghong NiuShanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, China.
Fei WangShanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, China.
Shumei XieShanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, China.
Fanyue SunDepartment of Reconstructive Sciences, Center for Regenerative Medicine and Developmental Biology, University of Connecticut Health Center, Farmington, CT, 06030, USA.
Ze WangShanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, China.
Maoxiao PengShanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, China.
Jiale LiShanghai Engineering Research Center of Aquaculture and College of Fisheries and Life Science, Shanghai Ocean University, 999 Hucheng Huan Road, Shanghai, 201306, China. jlli2009@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The razor clam Sinonovacula constricta is an important commercial species. The deficiency of developmental transcriptomic data is becoming the bottleneck of further researches on the mechanisms underlying settlement and metamorphosis in early development. In this study, de novo transcriptome sequencing was performed for S. constricta at different early developmental stages by using Illumina HiSeq 2000 paired-end (PE) sequencing technology. A total of 112,209,077 PE clean reads were generated. De novo assembly generated 249,795 contigs with an average length of 585 bp. Gene annotation resulted in the identification of 22,870 unigene hits against the NCBI database. Eight unique sequences related to metamorphosis were identified and analyzed using real-time PCR. The razor clam reference transcriptome would provide useful information on early developmental and metamorphosis mechanisms and could be used in the genetic breeding of shellfish.

Indexed as

Gene Expression Regulation, DevelopmentalMolecular Sequence AnnotationTranscriptomeAnimalsBivalviaDatabases, GeneticGene Expression ProfilingGene OntologyHigh-Throughput Nucleotide SequencingLarvaMetamorphosis, BiologicalPhylogenyReal-Time Polymerase Chain ReactionDevelopmentLarval metamorphosisSinonovacula constrictaTranscriptome

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What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.