ArticleMolecular and cellular biochemistry2016
Hypoxia inhibits mesenchymal stem cell proliferation through HIF1α-dependent regulation of P27.
Article in Molecular and cellular biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Hypoxia Promotes the Stemness of Mesangiogenic Progenitor Cells and Prevents Osteogenic but not Angiogenic Differentiation.Stem cell reviews and reports · 2024Article
- HypoxamiR-210-3p regulates mesenchymal stem cells proliferation via P53 & Akt.Molecular and cellular biochemistry · 2024Article
- [Protective effects of 2-methoxyestradiol against hypoxic pulmonary hypertension in neonatal rats].Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics · 2024Article
- Comprehensive assessment of goat adipose tissue-derived mesenchymal stem cells cultured in different media.Scientific reports · 2024Article
- An antioxidant response element regulates the HIF1α axis in breast cancer cells.Free radical biology & medicine · 2023Article
- Sodium selenite preserves rBM-MSCs' stemness, differentiation potential, and immunophenotype and protects them against oxidative stress via activation of the Nrf2 signaling pathway.BMC complementary medicine and therapies · 2023Article
- Review
- Expression of proliferation-related genes in BM-MSC-treated ALL cells in hypoxia condition is regulated under the influence of epigenetic factors in-vitro.Medical oncology (Northwood, London, England) · 2022Article
- Hypoxia Induces DPSC Differentiation versus a Neurogenic Phenotype by the Paracrine Mechanism.Biomedicines · 2022Article
- Ferroptosis and Tumor Drug Resistance: Current Status and Major Challenges.Frontiers in pharmacology · 2022Review
- The Proliferation and Stemness of Peripheral Blood-Derived Mesenchymal Stromal Cells Were Enhanced by Hypoxia.Frontiers in endocrinology · 2022Article
- Hypoxia-inducible factor-1α-mediated upregulation of CD99 promotes the proliferation of placental mesenchymal stem cells by regulating ERK1/2.World journal of stem cells · 2021Article
- Multilineage Differentiation Potential of Human Dental Pulp Stem Cells-Impact of 3D and Hypoxic Environment on Osteogenesis In Vitro.International journal of molecular sciences · 2020Article
- Article
- Succinate Supplement Elicited "Pseudohypoxia" Condition to Promote Proliferation, Migration, and Osteogenesis of Periodontal Ligament Cells.Stem cells international · 2020Article
- Microenvironment-Derived FGF-2 Stimulates Renal Cell Carcinoma Cell Proliferation through Modulation of p27Kip1: Implications for Spatial Niche Formation and Functional Intratumoral Heterogeneity.Pathobiology : journal of immunopathology, molecular and cellular biology · 2020Article
- Cross talk between 26S proteasome and mitochondria in human mesenchymal stem cells' ability to survive under hypoxia stress.The journal of physiological sciences : JPS · 2019Article
- Review
- Hypoxic culture enhances the expansion of rat bone marrow-derived mesenchymal stem cells via the regulatory pathways of cell division and apoptosis.In vitro cellular & developmental biology. Animal · 2018Article
- Comparative Study onStem cells international · 2018Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Stem cells have inherent properties of self-renewal and differentiation. High percentage of transplanted stem cells (95 %) die into ischemic heart due to unfavorable environment where in hypoxia considered among major contributing factors. Hypoxia inducible factor-1 α is a well-known transcription factor which robustly induced during hypoxia and an essential factor for adaptation under lower oxygen tension. The effects of hypoxia onto stem cells and its cell cycle are poorly understood. Mesenchymal stem cells were isolated from adult male Fischer-344 rats bone marrow and kept under hypoxia (1 % O2). Cell survival and proliferation were studied using MTT and CFSE assay which showed reduced proliferation rate with an arrest in G0/G1 phase of cell cycle using flow cytometry. Western blot analysis revealed an increase in expressions of HIF-1α along with P53, a tumor suppressor gene. Cyclin-dependent kinase inhibitor (CDKI), p27 was significantly increased, in concordance with the findings, cyclin D1 was also reduced under hypoxia and forestalling S phase entry. In addition, loss of function study with HIF-1α knockdown revealed progression of cell cycle even under hypoxia. Knocking down p27 abrogated the hypoxia-induced G1 checkpoint, suggesting a key regulator of G1/S transition in hypoxic cells. Hypoxia could cause HIF-1α-dependent increase in the expression of p27 leading to cell cycle arrest in G0/G1 phase. The demonstration of the molecular mechanism of hypoxia-induced G1/S regulation provides insight into a fundamental response of stem cells to low oxygen tension.
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