ArticleJournal of cell science2016
Conditional mutation of Smc5 in mouse embryonic stem cells perturbs condensin localization and mitotic progression.
Article in Journal of cell science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 31 citations in OpenAlex.
- Approaches for identification of 5' UTR mutations impacting translation and protein production from neurodevelopmental disorder genes.Cell reports methods · 2025Article
- Review
- NFATC2IP is a mediator of SUMO-dependent genome integrity.Genes & development · 2024Article
- SMC5/6 Promotes Replication Fork Stability via Negative Regulation of the COP9 Signalosome.International journal of molecular sciences · 2024Article
- Article
- In-frame deletion of SMC5 related with the phenotype of primordial dwarfism, chromosomal instability and insulin resistance.Clinical and translational medicine · 2023Article
- Pathogenic variants in SLF2 and SMC5 cause segmented chromosomes and mosaic variegated hyperploidy.Nature communications · 2022Article
- Explore a novel function of human condensins in cellular senescence.Cell & bioscience · 2020Review
- Adaptation of the AID system for stem cell and transgenic mouse research.Stem cell research · 2020Article
- SMC5/6 is required for replication fork stability and faithful chromosome segregation during neurogenesis.eLife · 2020Article
- Interaction between NSMCE4A and GPS1 links the SMC5/6 complex to the COP9 signalosome.BMC molecular and cell biology · 2020Article
- A genome-wide RNAi screen identifies the SMC5/6 complex as a non-redundant regulator of a Topo2a-dependent G2 arrest.Nucleic acids research · 2019Article
- Article
- Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts.Methods in molecular biology (Clifton, N.J.) · 2019Article
- SMC5/6: Multifunctional Player in Replication.Genes · 2018Review
- A Topology-Centric View on Mitotic Chromosome Architecture.International journal of molecular sciences · 2017Review
- SMC5/6 is required for the formation of segregation-competent bivalent chromosomes during meiosis I in mouse oocytes.Development (Cambridge, England) · 2017Article
- Condensin, master organizer of the genome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2017Review
- Non-SMC Element 2 (NSMCE2) of the SMC5/6 Complex Helps to Resolve Topological Stress.International journal of molecular sciences · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Correct duplication of stem cell genetic material and its appropriate segregation into daughter cells are requisites for tissue, organ and organism homeostasis. Disruption of stem cell genomic integrity can lead to developmental abnormalities and cancer. Roles of the Smc5/6 structural maintenance of chromosomes complex in pluripotent stem cell genome maintenance have not been investigated, despite its important roles in DNA synthesis, DNA repair and chromosome segregation as evaluated in other model systems. Using mouse embryonic stem cells (mESCs) with a conditional knockout allele of Smc5, we showed that Smc5 protein depletion resulted in destabilization of the Smc5/6 complex, accumulation of cells in G2 phase of the cell cycle and apoptosis. Detailed assessment of mitotic mESCs revealed abnormal condensin distribution and perturbed chromosome segregation, accompanied by irregular spindle morphology, lagging chromosomes and DNA bridges. Mutation of Smc5 resulted in retention of Aurora B kinase and enrichment of condensin on chromosome arms. Furthermore, we observed reduced levels of Polo-like kinase 1 at kinetochores during mitosis. Our study reveals crucial requirements of the Smc5/6 complex during cell cycle progression and for stem cell genome maintenance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.