ArticleJournal of orthopaedic research : official publication of the Orthopaedic Research Society2016
Location-dependent heterotopic ossification in the rat model: The role of activated matrix metalloproteinase 9.
Article in Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Comprehensive analysis of mRNA-microRNA-lncRNA expression profiles in post-traumatic elbow heterotopic ossification using RNA sequencing and experimental validation.Annals of medicine · 2026Article
- Giant calcific insertional Achilles tendinopathy with Haglund's deformity following childhood tendon lengthening: a rare case report.BMC musculoskeletal disorders · 2025Article
- Actionable disease insights from bedside-to-bench investigation in fibrodysplasia ossificans progressiva.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2024Article
- Matrix metalloproteinase-9 deficiency confers resilience in fibrodysplasia ossificans progressiva in a man and mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2024Article
- Cell Senescence in Heterotopic Ossification.Biomolecules · 2024Review
- Crosstalk between Bone and Nerves within Bone.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Review
- Metformin attenuates trauma-induced heterotopic ossification via inhibition of Bone Morphogenetic Protein signalling.Journal of cellular and molecular medicine · 2020Article
- Heterotopic ossification of tendon and ligament.Journal of cellular and molecular medicine · 2020Review
- Inhibition of immune checkpoints prevents injury-induced heterotopic ossification.Bone research · 2019Article
- Review
- Trauma-Induced Heterotopic Ossification Regulates the Blood-Nerve Barrier.Frontiers in neurology · 2018Review
- Progenitors in Peripheral Nerves Launch Heterotopic Ossification.Stem cells translational medicine · 2017Article
- Encapsulation of Adenovirus BMP2-Transduced Cells with PEGDA Hydrogels Allows Bone Formation in the Presence of Immune Response.Tissue engineering. Part A · 2017Article
- Inhibition of connexin 43 prevents trauma-induced heterotopic ossification.Scientific reports · 2016Article
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Authors and funding
11 authors at 3 institutions in 1 country.
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Abstract
Extremity amputation or traumatic injury can often lead to the formation of heterotopic ossification (HO). Studies to induce HO in rat muscle using cell-based gene therapy show that this process appears to be location dependent. In the present study, HO was induced in mice and rats through injection of immunologically matched cells transduced with either a replication-defective adenovirus possessing bone morphogenetic protein 2 (BMP2) or an empty adenovirus vector (control). Injection in rat near the skeletal bone resulted in HO, whereas cells injected into the same muscle group but distal from the bone did not result in bone formation. When cells were injected in the same limb at both locations at the same time, HO was formed at both sites. Characterization of the bone formation in rats versus mice demonstrated that different sources of osteogenic progenitors were involved, which may account for the location dependent bone formation observed in the rat. Further experimentation has shown that a potential reason for this difference may be the inability of rat to activate matrix metalloproteinase 9 (MMP9), an essential protease in mice necessary for recruitment of progenitors. Inhibition of active MMP9 in mice led to a significant decrease in HO. The studies reported here provide insight into the mechanisms and pathways leading to bone formation in different animals and species. It appears that not all animal models are appropriate for testing HO therapies, and our studies also challenge the conventional wisdom that larger animal models are better for testing treatments affecting bone. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:1894-1904, 2016.
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