Evidence map›Paper›PMID 26918945›Full record

ArticlePloS one2016

Magnetic Beads Enhance Adhesion of NIH 3T3 Fibroblasts: A Proof-of-Principle In Vitro Study for Implant-Mediated Long-Term Drug Delivery to the Inner Ear.

Pooyan Aliuos, Jennifer Schulze, Markus Schomaker, Günter Reuter, Stefan R O Stolle, Darja Werner, Tammo Ripken, Thomas Lenarz, Athanasia Warnecke

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 36% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Pooyan AliuosDepartment of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
Jennifer SchulzeDepartment of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
Markus SchomakerLaser Zentrum Hannover e.V., Hannover, Germany.
Günter ReuterDepartment of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
Stefan R O StolleDepartment of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
Darja WernerDepartment of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
Tammo RipkenCluster of Excellence "Hearing4All", Hannover, Germany.
Thomas LenarzDepartment of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
Athanasia WarneckeDepartment of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
Medizinische Hochschule Hannover · DEHearing4all · DELaser Zentrum Hannover · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionLong-term drug delivery to the inner ear may be achieved by functionalizing cochlear implant (CI) electrodes with cells providing neuroprotective factors. However, effective strategies in order to coat implant surfaces with cells need to be developed. Our vision is to make benefit of electromagnetic field attracting forces generated by CI electrodes to bind BDNF-secreting cells that are labelled with magnetic beads (MB) onto the electrode surfaces. Thus, the effect of MB-labelling on cell viability and BDNF production were investigated. MATERIALS AND

methodsMurine NIH 3T3 fibroblasts-genetically modified to produce BDNF-were labelled with MB.

resultsAtomic force and bright field microscopy illustrated the internalization of MB by fibroblasts after 24 h of cultivation. Labelling cells with MB did not expose cytotoxic effects on fibroblasts and allowed adhesion on magnetic surfaces with sufficient BDNF release. DISCUSSION: Our data demonstrate a novel approach for mediating enhanced long-term adhesion of BDNF-secreting fibroblasts on model electrode surfaces for cell-based drug delivery applications in vitro and in vivo. This therapeutic strategy, once transferred to cells suitable for clinical application, may allow the biological modifications of CI surfaces with cells releasing neurotrophic or other factors of interest.

Indexed as

Cochlear ImplantsDrug Delivery SystemsDrug ImplantsAnimalsBrain-Derived Neurotrophic FactorCell SurvivalEar, InnerMagneticsMiceNIH 3T3 CellsBrain-Derived Neurotrophic FactorDrug Implants

Identifiers

PMID26918945
PMCPMC4769079
OpenAlexW2284884795

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.