Evidence map›Paper›PMID 26917685›Full record

ArticlePlant physiology2016

HsfA2 Controls the Activity of Developmentally and Stress-Regulated Heat Stress Protection Mechanisms in Tomato Male Reproductive Tissues.

Sotirios Fragkostefanakis, Anida Mesihovic, Stefan Simm, Marine Josephine Paupière, Yangjie Hu, Puneet Paul, Shravan Kumar Mishra, Bettina Tschiersch, Klaus Theres, Arnaud Bovy and 2 more

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In one paragraph

Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers.

0numbers the graph read from it
0cells of the map it votes in
113citing papers in PubMed
7.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

113 citing papers in PubMed, 229 citations in OpenAlex.

  1. Review
  2. Article
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  7. Investigating cis-regulatory elements and gene expression in multiple tomato varieties using interpretable deep learning.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025
    Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Priming thermotolerance: unlocking heat resilience for climate-smart crops.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025
    Review
  14. Article
  15. Article
  16. Review
  17. Review
  18. Article
  19. Whole-genome identification ofFrontiers in plant science · 2025
    Article
  20. Genome-Wide Analysis of the Hsf Gene Family inInternational journal of molecular sciences · 2024
    Article

53 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 2 countries.

Sotirios FragkostefanakisDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.).ORCID 0000-0001-5311-7868
Anida MesihovicDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.).ORCID 0000-0001-6277-5141
Stefan SimmDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.).
Marine Josephine PaupièreDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.).
Yangjie HuDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.).ORCID 0000-0002-0910-6384
Puneet PaulDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.).ORCID 0000-0001-8220-8021
Shravan Kumar MishraDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.).ORCID 0000-0003-3899-0495
Bettina TschierschDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.).ORCID 0000-0002-6956-8804
Klaus TheresDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.).
Arnaud BovyDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.).
Enrico SchleiffDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.) schleiff@bio.uni-frankfurt.de scharf@bio.uni-frankfurt.de.
Klaus-Dieter ScharfDepartment of Biosciences, Molecular Cell Biology of Plants, Goethe University, D-60438 Frankfurt am Main, Germany (S.F., A.M., S.S., Y.H., P.P., S.K.M., E.S., K.-D.S.);Cluster of Excellence Frankfurt, Goethe University, D-60438 Frankfurt am Main, Germany (S.S., E.S.);Plant Breeding, Wageningen University, Wageningen 6708PB, The Netherlands (M.J.P., A.B.);Leibniz Institute of Plant Biochemistry, D-06120 Halle (Saale), Germany (B.T., K.-D.S.);Max Planck Institute for Plant Breeding Research, D-50829 Cologne, Germany (K.T.); andBuchmann Institute for Molecular Life Sciences, Goethe University, D-60438 Frankfurt am Main, Germany (E.S.) schleiff@bio.uni-frankfurt.de scharf@bio.uni-frankfurt.de.
Goethe University Frankfurt · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Male reproductive tissues are more sensitive to heat stress (HS) compared to vegetative tissues, but the basis of this phenomenon is poorly understood. Heat stress transcription factors (Hsfs) regulate the transcriptional changes required for protection from HS In tomato (Solanum lycopersicum), HsfA2 acts as coactivator of HsfA1a and is one of the major Hsfs accumulating in response to elevated temperatures. The contribution of HsfA2 in heat stress response (HSR) and thermotolerance was investigated in different tissues of transgenic tomato plants with suppressed HsfA2 levels (A2AS). Global transcriptome analysis and immunodetection of two major Hsps in vegetative and reproductive tissues showed that HsfA2 regulates subsets of HS-induced genes in a tissue-specific manner. Accumulation of HsfA2 by a moderate HS treatment enhances the capacity of seedlings to cope with a subsequent severe HS, suggesting an important role for HsfA2 in regulating acquired thermotolerance. In pollen, HsfA2 is an important coactivator of HsfA1a during HSR HsfA2 suppression reduces the viability and germination rate of pollen that received the stress during the stages of meiosis and microspore formation but had no effect on more advanced stages. In general, pollen meiocytes and microspores are characterized by increased susceptibility to HS due to their lower capacity to induce a strong HSR This sensitivity is partially mitigated by the developmentally regulated expression of HsfA2 and several HS-responsive genes mediated by HsfA1a under nonstress conditions. Thereby, HsfA2 is an important factor for the priming process that sustains pollen thermotolerance during microsporogenesis.

Indexed as

Gene Expression Regulation, PlantHeat-Shock ResponseDNA-Binding ProteinsFlowersGametogenesis, PlantGene Expression ProfilingGene Expression Regulation, DevelopmentalHeat-Shock ProteinsHeat Shock Transcription FactorsHot TemperatureOrgan SpecificityPlant LeavesPlant ProteinsPlants, Genetically ModifiedPollenSeedlingsDNA-Binding ProteinsHeat-Shock ProteinsHeat Shock Transcription FactorsPlant ProteinsTranscription Factors

Identifiers

PMID26917685
PMCPMC4825147
OpenAlexW2285317814

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