ArticlePlant physiology2016
Composition, Assembly, and Trafficking of a Wheat Xylan Synthase Complex.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- Cell wall-related glycosyltransferases and wall architecture in the model liverwort Marchantia polymorpha.The Plant journal : for cell and molecular biology · 2025Article
- Enzymatic characterization and docking simulation of a xylan synthase catalytic subunit,Plant biotechnology (Tokyo, Japan) · 2025Article
- Multiprotein Complexes of Plant Glycosyltransferases Involved in Their Function and Trafficking.Plants (Basel, Switzerland) · 2025Review
- XYLAN O-ACETYLTRANSFERASE 6 promotes xylan synthesis by forming a complex with IRX10 and governs wall formation in rice.The Plant cell · 2024Article
- Genetic dissection of monosaccharides contents in rice whole grain using genome-wide association study.The plant genome · 2023Article
- Genetic Approaches to Increase Arabinoxylan and β-Glucan Content in Wheat.Plants (Basel, Switzerland) · 2023Review
- Impact of xylan on field productivity and wood saccharification properties in aspen.Frontiers in plant science · 2023Article
- Critical Determinants in ER-Golgi Trafficking of Enzymes Involved in Glycosylation.Plants (Basel, Switzerland) · 2022Review
- β-1,4-Xylan backbone synthesis in higher plants: How complex can it be?Frontiers in plant science · 2022Article
- Identification and expression analysis of the glycosyltransferase GT43 family members in bamboo reveal their potential function in xylan biosynthesis during rapid growth.BMC genomics · 2021Article
- Polysaccharide Biosynthesis: Glycosyltransferases and Their Complexes.Frontiers in plant science · 2021Article
- Article
- BdGT43B2 functions in xylan biosynthesis and is essential for seedling survival inPlant direct · 2020Article
- A dominant negative approach to reduce xylan in plants.Plant biotechnology journal · 2020Article
- Organization of Xylan Production in the Golgi During Secondary Cell Wall Biosynthesis.Plant physiology · 2019Article
- Poplar carbohydrate-active enzymes: whole-genome annotation and functional analyses based on RNA expression data.The Plant journal : for cell and molecular biology · 2019Article
- Xylan in the Middle: Understanding Xylan Biosynthesis and Its Metabolic Dependencies Toward Improving Wood Fiber for Industrial Processing.Frontiers in plant science · 2019Review
- Critical Review of Plant Cell Wall Matrix Polysaccharide Glycosyltransferase Activities Verified by Heterologous Protein Expression.Frontiers in plant science · 2019Review
- A two-phase model for the non-processive biosynthesis of homogalacturonan polysaccharides by the GAUT1:GAUT7 complex.The Journal of biological chemistry · 2018Article
- Genome-wide identification and analysis of the ALTERNATIVE OXIDASE gene family in diploid and hexaploid wheat.PloS one · 2018Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Xylans play an important role in plant cell wall integrity and have many industrial applications. Characterization of xylan synthase (XS) complexes responsible for the synthesis of these polymers is currently lacking. We recently purified XS activity from etiolated wheat (Triticum aestivum) seedlings. To further characterize this purified activity, we analyzed its protein composition and assembly. Proteomic analysis identified six main proteins: two glycosyltransferases (GTs) TaGT43-4 and TaGT47-13; two putative mutases (TaGT75-3 and TaGT75-4) and two non-GTs; a germin-like protein (TaGLP); and a vernalization related protein (TaVER2). Coexpression of TaGT43-4, TaGT47-13, TaGT75-3, and TaGT75-4 in Pichia pastoris confirmed that these proteins form a complex. Confocal microscopy showed that all these proteins interact in the endoplasmic reticulum (ER) but the complexes accumulate in Golgi, and TaGT43-4 acts as a scaffold protein that holds the other proteins. Furthermore, ER export of the complexes is dependent of the interaction between TaGT43-4 and TaGT47-13. Immunogold electron microscopy data support the conclusion that complex assembly occurs at specific areas of the ER before export to the Golgi. A di-Arg motif and a long sequence motif within the transmembrane domains were found conserved at the NH2-terminal ends of TaGT43-4 and homologous proteins from diverse taxa. These conserved motifs may control the forward trafficking of the complexes and their accumulation in the Golgi. Our findings indicate that xylan synthesis in grasses may involve a new regulatory mechanism linking complex assembly with forward trafficking and provide new insights that advance our understanding of xylan biosynthesis and regulation in plants.
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