Evidence map›Paper›PMID 26917237›Full record

ArticleNeuro-oncology2016

Glycolysis and the pentose phosphate pathway are differentially associated with the dichotomous regulation of glioblastoma cell migration versus proliferation.

Annegret Kathagen-Buhmann, Alexander Schulte, Jonathan Weller, Mareike Holz, Christel Herold-Mende, Rainer Glass, Katrin Lamszus

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neuro-oncology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
87citing papers in PubMed, 1 pooled it
4.7field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

87 citing papers in PubMed, 1 synthesis or guideline pooled it, 146 citations in OpenAlex.

  1. Physical Forces in Glioblastoma Migration: A Systematic Review.International journal of molecular sciences · 2022
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  18. Glycolytic metabolism: Food for immune cells, fuel for depression?Brain, behavior, & immunity - health · 2024
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27 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Annegret Kathagen-BuhmannDepartment of Neurosurgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany (A.K.-B., A.S., J.W., M.H., K.L.); Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany (C.H.-M.); Department of Neurosurgery, Klinikum Grosshadern, Ludwigs-Maximilians-University, Munich, Germany (R.G.).
Alexander SchulteDepartment of Neurosurgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany (A.K.-B., A.S., J.W., M.H., K.L.); Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany (C.H.-M.); Department of Neurosurgery, Klinikum Grosshadern, Ludwigs-Maximilians-University, Munich, Germany (R.G.).
Jonathan WellerDepartment of Neurosurgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany (A.K.-B., A.S., J.W., M.H., K.L.); Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany (C.H.-M.); Department of Neurosurgery, Klinikum Grosshadern, Ludwigs-Maximilians-University, Munich, Germany (R.G.).
Mareike HolzDepartment of Neurosurgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany (A.K.-B., A.S., J.W., M.H., K.L.); Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany (C.H.-M.); Department of Neurosurgery, Klinikum Grosshadern, Ludwigs-Maximilians-University, Munich, Germany (R.G.).
Christel Herold-MendeDepartment of Neurosurgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany (A.K.-B., A.S., J.W., M.H., K.L.); Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany (C.H.-M.); Department of Neurosurgery, Klinikum Grosshadern, Ludwigs-Maximilians-University, Munich, Germany (R.G.).
Rainer GlassDepartment of Neurosurgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany (A.K.-B., A.S., J.W., M.H., K.L.); Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany (C.H.-M.); Department of Neurosurgery, Klinikum Grosshadern, Ludwigs-Maximilians-University, Munich, Germany (R.G.).
Katrin LamszusDepartment of Neurosurgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany (A.K.-B., A.S., J.W., M.H., K.L.); Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, Heidelberg, Germany (C.H.-M.); Department of Neurosurgery, Klinikum Grosshadern, Ludwigs-Maximilians-University, Munich, Germany (R.G.) lamszus@uke.de.
Heidelberg University · DELudwig-Maximilians-Universität München · DEUniversität Hamburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe dichotomy between glioblastoma cell migration and proliferation is regulated by various parameters including oxygen tension. In glioblastoma stem-like cells, hypoxia induces downregulation of pentose phosphate pathway (PPP) enzymes and a flux shift towards glycolysis. We investigated whether the 2 parallel glucose metabolic pathways are intrinsically linked with cell function and whether these pathways are mechanistically involved in regulating functional programs.

methodsEnzyme expression, migration, and proliferation under hypoxia were studied in multiple cell types. Rapidly and slowly dividing or migrating glioblastoma cells were separated, and enzyme profiles were compared. Glucose-6-phosphate dehydrogenase (G6PD) and Aldolase C (ALDOC), the most strongly inversely regulated PPP and glycolysis enzymes, were knocked down by short hairpin RNA.

resultsHypoxia caused downregulation of PPP enzymes and upregulation of glycolysis enzymes in a broad spectrum of cancer and nonneoplastic cells and consistently stimulated migration while reducing proliferation. PPP enzyme expression was increased in rapidly dividing glioblastoma cells, whereas glycolysis enzymes were decreased. Conversely, glycolysis enzymes were elevated in migrating cells, whereas PPP enzymes were diminished. Knockdown of G6PD reduced glioblastoma cell proliferation, whereas ALDOC knockdown decreased migration. Enzyme inhibitors had similar effects. G6PD knockdown in a highly proliferative but noninvasive glioblastoma cell line resulted in prolonged survival of mice with intracerebral xenografts, whereas ALDOC knockdown shortened survival. In a highly invasive glioblastoma xenograft model, tumor burden was unchanged by either knockdown.

conclusionsCell function and metabolic state are coupled independently of hypoxia, and glucose metabolic pathways are causatively involved in regulating "go or grow" cellular programs.

Indexed as

Cell MovementCell ProliferationPentose Phosphate PathwayAnimalsApoptosisGlioblastomaGlucoseGlycolysisHumansHypoxiaMiceMice, HairlessMice, Inbred NODMice, SCIDTumor Cells, CulturedXenograft Model Antitumor AssaysGlucosegliomaglycolysisinvasionmetabolismpentose phosphate pathway

Identifiers

PMID26917237
PMCPMC4998991
OpenAlexW2275174502

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.