ArticleJournal of the American Chemical Society2016
Supramolecular Probes for Assessing Glutamine Uptake Enable Semi-Quantitative Metabolic Models in Single Cells.
Article in Journal of the American Chemical Society, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 38 citations in OpenAlex.
- Non-Mass Spectrometric Targeted Single-Cell Metabolomics.Trends in analytical chemistry : TRAC · 2023Article
- Crosstalk between COVID-19 Infection and Kidney Diseases: A Review on the Metabolomic Approaches.Vaccines · 2023Review
- The Intriguing Landscape of Single-Cell Protein Analysis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- Integrated analysis of plasma and single immune cells uncovers metabolic changes in individuals with COVID-19.Nature biotechnology · 2022Article
- Glutamine Imaging: A New Avenue for Glioma Management.AJNR. American journal of neuroradiology · 2022Review
- Constraint-Based Reconstruction and Analyses of Metabolic Models: Open-Source Python Tools and Applications to Cancer.Frontiers in oncology · 2022Review
- Single-cell profiling of D-2-hydroxyglutarate using surface-immobilized resazurin analogs.Biosensors & bioelectronics · 2021Article
- A guide to interrogating immunometabolism.Nature reviews. Immunology · 2021Review
- Single-Cell Profiling of Fatty Acid Uptake Using Surface-Immobilized Dendrimers.Journal of the American Chemical Society · 2021Article
- The Development of Single-Cell Metabolism and Its Role in Studying Cancer Emergent Properties.Frontiers in oncology · 2021Review
- Metabolic Reprogramming of Immune Cells at the Maternal-Fetal Interface and the Development of Techniques for Immunometabolism.Frontiers in immunology · 2021Review
- Multi-omic single-cell snapshots reveal multiple independent trajectories to drug tolerance in a melanoma cell line.Nature communications · 2020Article
- Surface Immobilization of Redox-Labile Fluorescent Probes: Enabling Single-Cell Co-Profiling of Aerobic Glycolysis and Oncogenic Protein Signaling Activities.Angewandte Chemie (International ed. in English) · 2018Article
- Defining Cell Identity with Single-Cell Omics.Proteomics · 2018Review
- Supramolecular Host-Guest Chemistry-Based Folate/Riboflavin Functionalization and Cancer Cell Labeling of Nanoparticles.ACS omega · 2017Article
- Fluorescence Resonance Energy Transfer Systems in Supramolecular Macrocyclic Chemistry.Molecules (Basel, Switzerland) · 2017Review
- Single cell proteomics in biomedicine: High-dimensional data acquisition, visualization, and analysis.Proteomics · 2017Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We describe a supramolecular surface competition assay for quantifying glutamine uptake from single cells. Cy3-labeled cyclodextrins were immobilized on a glass surface as a supramolecular host/FRET donor, and adamantane-BHQ2 conjugates were employed as the guest/quencher. An adamantane-labeled glutamine analog was selected through screening a library of compounds and validated by cell uptake experiments. When integrated onto a single cell barcode chip with a multiplex panel of 15 other metabolites, associated metabolic enzymes, and phosphoproteins, the resultant data provided input for a steady-state model that describes energy potential in single cells and correlates that potential with receptor tyrosine kinase signaling. We utilize this integrated assay to interrogate a dose-dependent response of model brain cancer cells to EGFR inhibition. We find that low-dose (1 μM erlotinib) drugging actually increases cellular energy potential even as glucose uptake and phosphoprotein signaling is repressed. We also identify new interactions between phosphoprotein signaling and cellular energy processes that may help explain the facile resistance exhibited by certain cancer patients to EGFR inhibitors.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.