ArticleScientific reports2016
Generation and characterization of rat liver stem cell lines and their engraftment in a rat model of liver failure.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Liver organoids: From fabrication to application in liver diseases.Frontiers in physiology · 2022Pooled it
- Construction of a Duck Intestinal Organoid Culture System: From Crypt Isolation to Medium Optimization.Animals : an open access journal from MDPI · 2025Article
- Review
- Research Progress on Hepatitis E Virus Culture.Pathogens (Basel, Switzerland) · 2025Review
- Cellular and transcriptional heterogeneity in the intrahepatic biliary epithelium.Gastro hep advances · 2023Article
- Liver organoids: an in vitro 3D model for liver cancer study.Cell & bioscience · 2022Review
- Syngeneic implantation of mouse hepatic progenitor cell-derived three-dimensional liver tissue with dense collagen fibrils.World journal of gastroenterology · 2022Article
- Human Endometrial Organoids: Recent Research Progress and Potential Applications.Frontiers in cell and developmental biology · 2022Review
- Precancerous liver diseases do not cause increased mutagenesis in liver stem cells.Communications biology · 2021Article
- Survival-Assured Liver Injury Preconditioning (SALIC) Enables Robust Expansion of Human Hepatocytes in FahAdvanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Cell-Based Regeneration and Treatment of Liver Diseases.International journal of molecular sciences · 2021Review
- Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models.Frontiers in genetics · 2021Review
- A Chemically Defined Hydrogel for Human Liver Organoid Culture.Advanced functional materials · 2020Article
- Creating rat hepatocyte organoid as anWorld journal of stem cells · 2020Article
- Review
- Production of Human Pluripotent Stem Cell-Derived Hepatic Cell Lineages and Liver Organoids: Current Status and Potential Applications.Bioengineering (Basel, Switzerland) · 2020Review
- Novel alternative transplantation therapy for orthotopic liver transplantation in liver failure: A systematic review.World journal of transplantation · 2020Article
- Article
- Review
- Towards Bioengineered Liver Stem Cell Transplantation Studies in a Preclinical Dog Model for Inherited Copper Toxicosis.Bioengineering (Basel, Switzerland) · 2019Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
Abstract
The rat is an important model for liver regeneration. However, there is no in vitro culture system that can capture the massive proliferation that can be observed after partial hepatectomy in rats. We here describe the generation of rat liver stem cell lines. Rat liver stem cells, which grow as cystic organoids, were characterized by high expression of the stem cell marker Lgr5, by the expression of liver progenitor and duct markers, and by low expression of hepatocyte markers, oval cell markers, and stellate cell markers. Prolonged cultures of rat liver organoids depended on high levels of WNT-signalling and the inhibition of BMP-signaling. Upon transplantation of clonal lines to a Fah(-/-) Il2rg(-/-) rat model of liver failure, the rat liver stem cells engrafted into the host liver where they differentiated into areas with FAH and Albumin positive hepatocytes. Rat liver stem cell lines hold potential as consistent reliable cell sources for pharmacological, toxicological or metabolic studies. In addition, rat liver stem cell lines may contribute to the development of regenerative medicine in liver disease. To our knowledge, the here described liver stem cell lines represent the first organoid culture system in the rat.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.