ArticleAngewandte Chemie (International ed. in English)2016
Orthogonal Protein Assembly on DNA Nanostructures Using Relaxases.
Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 46 citations in OpenAlex.
- Homogeneous Antibody-DNA Conjugates Using Unmodified Oligonucleotides and Photo-Cross-Linkable Protein G-HUH Endonuclease Fusion Proteins.Bioconjugate chemistry · 2026Article
- Sequence-directed covalent protein-RNA linkages in a single step using engineered HUH-tags.Nucleic acids research · 2026Article
- Design and Characterization of a Gene-Encoding DNA Nanoparticle in a Cell-Free Transcription-Translation System.ACS applied nano materials · 2024Article
- The crystal structure of the human smacovirus 1 Rep domain.Acta crystallographica. Section F, Structural biology communications · 2023Article
- Functionalizing DNA origami to investigate and interact with biological systems.Nature reviews. Materials · 2023Article
- Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins.ACS nano · 2021Article
- Molecular underpinnings of ssDNA specificity by Rep HUH-endonucleases and implications for HUH-tag multiplexing and engineering.Nucleic acids research · 2021Article
- Quantitative Assessment of Tip Effects in Single-Molecule High-Speed Atomic Force Microscopy Using DNA Origami Substrates.Angewandte Chemie (International ed. in English) · 2020Article
- Nucleic Acid Hybrids as Advanced Antibacterial Nanocarriers.Pharmaceutics · 2020Review
- Rational design of a DNA sequence-specific modular protein tag by tuning the alkylation kinetics.Chemical science · 2019Article
- Nucleic Acid-Barcoding Technologies: Converting DNA Sequencing into a Broad-Spectrum Molecular Counter.Angewandte Chemie (International ed. in English) · 2019Review
- T7 RNA polymerase non-specifically transcribes and induces disassembly of DNA nanostructures.Nucleic acids research · 2018Article
- DNA Origami Route for Nanophotonics.ACS photonics · 2018Review
- Entirely enzymatic nanofabrication of DNA-protein conjugates.Nucleic acids research · 2017Article
- Sequence-Directed Covalent Protein-DNA Linkages in a Single Step Using HUH-Tags.Journal of the American Chemical Society · 2017Article
- Orthogonal Protein Assembly on DNA Nanostructures Using Relaxases.Angewandte Chemie (International ed. in English) · 2016Article
- Design of Novel Relaxase Substrates Based on Rolling Circle Replicases for Bioconjugation to DNA Nanostructures.PloS one · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA-binding proteins are promising reagents for the sequence-specific modification of DNA-based nanostructures. Here, we investigate the utility of a series of relaxase proteins-TrwC, TraI, and MobA-for nanofunctionalization. Relaxases are involved in the conjugative transfer of plasmids between bacteria, and bind to their DNA target sites via a covalent phosphotyrosine linkage. We study the binding of the relaxases to two standard DNA origami structures-rodlike six-helix bundles and flat rectangular origami sheets. We find highly orthogonal binding of the proteins with binding yields of 40-50 % per binding site, which is comparable to other functionalization methods. The yields differ for the two origami structures and also depend on the position of the binding sites. Due to their specificity for a single-stranded DNA target, their orthogonality, and their binding properties, relaxases are a uniquely useful addition to the toolbox available for the modification of DNA nanostructures with proteins.
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