ArticleMolecular plant pathology2017
Production of small cysteine-rich effector proteins in Escherichia coli for structural and functional studies.
Article in Molecular plant pathology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Cysteine-Rich Peptides in Food Systems: Structure, Sources, Functional Properties, Potential Applications, and Future Perspectives.Probiotics and antimicrobial proteins · 2026Review
- The root-knot nematode effector Mi1D08B targets a NINJA-family co-repressor to suppress jasmonate production and promote infection in soybean.The Plant journal : for cell and molecular biology · 2026Article
- A Fungal Cysteine-Rich Protein SsSCP Activates Immune Responses and Confers Broad-Spectrum Disease Resistance in Tomato.Plant biotechnology journal · 2026Article
- QTL detection and candidate gene analysis of grape white rot resistance by interspecific grape (Horticulture research · 2023Article
- TheJournal of fungi (Basel, Switzerland) · 2022Article
- Three types of genes underlying the Gametophyte factor1 locus cause unilateral cross incompatibility in maize.Nature communications · 2022Article
- Chemical synthesis ofRSC chemical biology · 2022Article
- Characterization of Salivary Secreted Proteins That Induce Cell Death FromFrontiers in plant science · 2022Article
- Simple and efficient heterologous expression of necrosis-inducing effectors using the model plantPlant direct · 2021Article
- Agroinfiltration for transient gene expression and characterisation of fungal pathogen effectors in cool-season grain legume hosts.Plant cell reports · 2021Article
- Cellular Dynamics of Transition Metal Exchange on Proteins: A Challenge but a Bonanza for Coordination Chemistry.Biomolecules · 2020Review
- Smut fungi as a stratagem to characterize rust effectors: opportunities and challenges.World journal of microbiology & biotechnology · 2020Review
- Structural genomics applied to the rust fungus Melampsora larici-populina reveals two candidate effector proteins adopting cystine knot and NTF2-like protein folds.Scientific reports · 2019Article
- Heterologous Expression of theFrontiers in microbiology · 2019Article
- Crystal structure of the Melampsora lini effector AvrP reveals insights into a possible nuclear function and recognition by the flax disease resistance protein P.Molecular plant pathology · 2018Article
- A Puccinia striiformis f. sp. tritici secreted protein activates plant immunity at the cell surface.Scientific reports · 2017Article
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8 authors.
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Abstract
Although the lifestyles and infection strategies of plant pathogens are diverse, a prevailing feature is the use of an arsenal of secreted proteins, known as effectors, which aid in microbial infection. In the case of eukaryotic filamentous pathogens, such as fungi and oomycetes, effector proteins are typically dissimilar, at the protein sequence level, to known protein families and functional domains. Consequently, we currently have a limited understanding of how fungal and oomycete effectors promote disease. Protein biochemistry and structural biology are two methods that can contribute greatly to the understanding of protein function. Both techniques are dependent on obtaining proteins that are pure and functional, and generally require the use of heterologous recombinant protein expression systems. Here, we present a general scheme and methodology for the production and characterization of small cysteine-rich (SCR) effectors utilizing Escherichia coli expression systems. Using this approach, we successfully produced cysteine-rich effectors derived from the biotrophic fungal pathogen Melampsora lini and the necrotrophic fungal pathogen Parastagonospora nodorum. Access to functional recombinant proteins facilitated crystallization and functional experiments. These results are discussed in the context of a general workflow that may serve as a template for others interested in understanding the function of SCR effector(s) from their plant pathogen(s) of interest.
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