Evidence map›Paper›PMID 26915109›Full record

ArticleNeurochemical research2016

A2B5+/GFAP+ Cells of Rat Spinal Cord Share a Similar Lipid Profile with Progenitor Cells: A Comparative Lipidomic Study.

Yutaka Itokazu, Nobuyoshi Tajima, Laura Kerosuo, Pentti Somerharju, Hannu Sariola, Robert K Yu, Reijo Käkelä

Open access · greenAbstract readComparative Study
PubMed Publisher
In one paragraph

Article in Neurochemical research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Gangliosides in Nerve Cell Specification.Progress in molecular biology and translational science · 2018
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Yutaka ItokazuDepartment of Biosciences, University of Helsinki, Biocenter 3, P.O. Box 65, 00014, Helsinki, Finland.
Nobuyoshi TajimaInstitute of Biomedicine, Department of Biochemistry and Developmental Biology, University of Helsinki, 00014, Helsinki, Finland.
Laura KerosuoInstitute of Biomedicine, Department of Biochemistry and Developmental Biology, University of Helsinki, 00014, Helsinki, Finland.
Pentti SomerharjuInstitute of Biomedicine, Department of Biochemistry and Developmental Biology, University of Helsinki, 00014, Helsinki, Finland.
Hannu SariolaInstitute of Biomedicine, Department of Biochemistry and Developmental Biology, University of Helsinki, 00014, Helsinki, Finland.
Robert K YuDepartment of Neuroscience and Regenerative Medicine, Medical College of Georgia, Augusta University, Augusta, GA, 30912, USA.
Reijo KäkeläDepartment of Biosciences, University of Helsinki, Biocenter 3, P.O. Box 65, 00014, Helsinki, Finland. reijo.kakela@helsinki.fi.
University of Helsinki · FIAugusta University · USKanazawa Medical University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The central nervous system (CNS) harbors multiple glial fibrillary acidic protein (GFAP) expressing cell types. In addition to the most abundant cell type of the CNS, the astrocytes, various stem cells and progenitor cells also contain GFAP+ populations. Here, in order to distinguish between two types of GFAP expressing cells with or without the expression of the A2B5 antigens, we performed lipidomic analyses on A2B5+/GFAP+ and A2B5-/GFAP+ cells from rat spinal cord. First, A2B5+/GFAP- progenitors were exposed to the leukemia inhibitory factor (LIF) or bone morphogenetic protein (BMP) to induce their differentiation to A2B5+/GFAP+ cells or A2B5-/GFAP+ astrocytes, respectively. The cells were then analyzed for changes in their phospholipid, sphingolipid or acyl chain profiles by mass spectrometry and gas chromatography. Compared to A2B5+/GFAP- progenitors, A2B5-/GFAP+ astrocytes contained higher amounts of ether phospholipids (especially the species containing arachidonic acid) and sphingomyelin, which may indicate characteristics of cellular differentiation and inability for multipotency. In comparison, principal component analyses revealed that the lipid composition of A2B5+/GFAP+ cells retained many of the characteristics of A2B5+/GFAP- progenitors, but their lipid profile was different from that of A2B5-/GFAP+ astrocytes. Thus, our study demonstrated that two GFAP+ cell populations have distinct lipid profiles with the A2B5+/GFAP+ cells sharing a phospholipid profile with progenitors rather than astrocytes. The progenitor cells may require regulated low levels of lipids known to mediate signaling functions in differentiated cells, and the precursor lipid profiles may serve as one measure of the differentiation capacity of a cell population.

Indexed as

AnimalsCells, CulturedGangliosidesGlial Fibrillary Acidic ProteinMembrane LipidsRatsSpinal CordStem Cellsganglioside A2B5GangliosidesGfap protein, ratGlial Fibrillary Acidic ProteinMembrane LipidsA2B5 antigenAstrocyteGlial fibrillary acidic proteinGlial progenitor/precursor cellLipidomicsMass spectrometryPhospholipidSphingolipid

Identifiers

PMID26915109
OpenAlexW2278040834

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.