Evidence map›Paper›PMID 26915106›Full record

ArticleNeurochemical research2016

Characterization of Amino Acid Profile and Enzymatic Activity in Adult Rat Astrocyte Cultures.

Débora Guerini Souza, Bruna Bellaver, Gisele Hansel, Bernardo Assein Arús, Gabriela Bellaver, Aline Longoni, Janaina Kolling, Angela T S Wyse, Diogo Onofre Souza, André Quincozes-Santos

Abstract read
PubMed Publisher
In one paragraph

Article in Neurochemical research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 6 citations in OpenAlex.

  1. Clozapine induces astrocyte-dependent FDG-PET hypometabolism.European journal of nuclear medicine and molecular imaging · 2022
    Article
  2. [Nature neuroscience · 2017
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Débora Guerini SouzaDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600, Anexo, PO Box: 90035-003, Porto Alegre, RS, Brazil. debsrs@gmail.com.
Bruna BellaverDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600, Anexo, PO Box: 90035-003, Porto Alegre, RS, Brazil.
Gisele HanselDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600, Anexo, PO Box: 90035-003, Porto Alegre, RS, Brazil.
Bernardo Assein ArúsDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600, Anexo, PO Box: 90035-003, Porto Alegre, RS, Brazil.
Gabriela BellaverDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600, Anexo, PO Box: 90035-003, Porto Alegre, RS, Brazil.
Aline LongoniDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600, Anexo, PO Box: 90035-003, Porto Alegre, RS, Brazil.
Janaina KollingDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600, Anexo, PO Box: 90035-003, Porto Alegre, RS, Brazil.
Angela T S WyseDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600, Anexo, PO Box: 90035-003, Porto Alegre, RS, Brazil.
Diogo Onofre SouzaDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600, Anexo, PO Box: 90035-003, Porto Alegre, RS, Brazil.
André Quincozes-SantosDepartamento de Bioquímica, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos, 2600, Anexo, PO Box: 90035-003, Porto Alegre, RS, Brazil.
Universidade Federal do Rio Grande do Sul · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Astrocytes are multitasking players in brain complexity, possessing several receptors and mechanisms to detect, participate and modulate neuronal communication. The functionality of astrocytes has been mainly unraveled through the study of primary astrocyte cultures, and recently our research group characterized a model of astrocyte cultures derived from adult Wistar rats. We, herein, aim to characterize other basal functions of these cells to explore the potential of this model for studying the adult brain. To characterize the astrocytic phenotype, we determined the presence of GFAP, GLAST and GLT 1 proteins in cells by immunofluorescence. Next, we determined the concentrations of thirteen amino acids, ATP, ADP, adenosine and calcium in astrocyte cultures, as well as the activities of Na(+)/K(+)-ATPase and acetylcholine esterase. Furthermore, we assessed the presence of the GABA transporter 1 (GAT 1) and cannabinoid receptor 1 (CB 1) in the astrocytes. Cells demonstrated the presence of glutamine, consistent with their role in the glutamate-glutamine cycle, as well as glutamate and D-serine, amino acids classically known to act as gliotransmitters. ATP was produced and released by the cells and ADP was consumed. Calcium levels were in agreement with those reported in the literature, as were the enzymatic activities measured. The presence of GAT 1 was detected, but the presence of CB 1 was not, suggesting a decreased neuroprotective capacity in adult astrocytes under in vitro conditions. Taken together, our results show cellular functionality regarding the astrocytic role in gliotransmission and neurotransmitter management since they are able to produce and release gliotransmitters and to modulate the cholinergic and GABAergic systems.

Indexed as

AcetylcholinesteraseAge FactorsAmino AcidsAnimalsAstrocytesCells, CulturedCerebral CortexChromatography, High Pressure LiquidGABA Plasma Membrane Transport ProteinsMaleRatsRats, WistarSodium-Potassium-Exchanging ATPaseAcetylcholinesteraseAmino AcidsGABA Plasma Membrane Transport ProteinsSodium-Potassium-Exchanging ATPaseAChEAdult astrocytesAmino acidsGliotransmittersNa+/K+-ATPase

Identifiers

PMID26915106
OpenAlexW2285298619

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.