Evidence map›Paper›PMID 26913720›Full record

ArticleClinical & experimental metastasis2016

MiR-193a-3p and miR-193a-5p suppress the metastasis of human osteosarcoma cells by down-regulating Rab27B and SRR, respectively.

Youguang Pu, Fangfang Zhao, Wenjing Cai, Xianghui Meng, Yinpeng Li, Shanbao Cai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical & experimental metastasis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
64citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

64 citing papers in PubMed, 2 syntheses or guidelines pooled it.

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4 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Youguang PuCancer Epigenetics Program, Anhui Cancer Hospital, Hefei, 230031, Anhui, China.
Fangfang ZhaoCancer Epigenetics Program, Anhui Cancer Hospital, Hefei, 230031, Anhui, China.
Wenjing CaiIndiana University School of Medicine, Indianapolis, IN, 46202, USA.
Xianghui MengDepartment of Orthopedic Surgery, Anhui Cancer Hospital, Hefei, 230031, Anhui, China.
Yinpeng LiXinxiang Medical University, Xinxiang, 453000, Henan, China.
Shanbao CaiCancer Epigenetics Program, Anhui Cancer Hospital, Hefei, 230031, Anhui, China. shanbaocai651116@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

MicroRNAs have been identified as key players in the development and progression of osteosarcoma, which is the most common primary malignancy of bone. Sequencing-based miR-omic and quantitative real-time PCR analyses suggested that the expression of miR-193a-3p and miR-193a-5p was decreased by DNA methylation at their promoter region in a highly metastatic osteosarcoma cell line (MG63.2) relative to their expression in the less metastatic MG63 cell line. Further wound-healing and invasion assays demonstrated that both miR-193a-3p and miR-193a-5p suppressed osteosarcoma cell migration and invasion. Moreover, introducing miR-193a-3p and miR-193a-5p mimics into MG63.2 cells or antagomiRs into MG63 cells confirmed their critical roles in osteosarcoma metastasis. Additionally, bioinformatics prediction along with biochemical assay results clearly suggested that the secretory small GTPase Rab27B and serine racemase (SRR) were direct targets of miR-193a-3p and miR-193a-5p, respectively. These two targets are indeed involved in the miR-193a-3p- and miR-193a-5p-induced suppression of osteosarcoma cell migration and invasion. MiR-193a-3p and miR-193a-5p play important roles in osteosarcoma metastasis through down-regulation of the Rab27B and SRR genes and therefore may serve as useful biomarkers for the diagnosis of osteosarcoma and as potential candidates for the treatment of metastatic osteosarcoma.

Indexed as

Biomarkers, TumorCell Line, TumorCell MovementDNA MethylationGene Expression Regulation, NeoplasticHigh-Throughput Nucleotide SequencingHumansMicroRNAsNeoplasm InvasivenessNeoplasm MetastasisOsteosarcomaPromoter Regions, Geneticrab GTP-Binding ProteinsRacemases and EpimerasesSerine RacemaseBiomarkers, TumorMicroRNAsMIRN193 microRNA, humanRab27B protein, humanrab GTP-Binding ProteinsRacemases and EpimerasesSerine RacemaseMetastasisMiR-193a-3p and miR-193a-5pOsteosarcomaRab27BSRR

Identifiers

PMID26913720
PMCPMC4799803

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.