ArticleAnimal biotechnology2016
New Rapid Method of DNA Isolation from Milk Somatic Cells.
Article in Animal biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 54 citations in OpenAlex.
- Development and evaluation of a real-time PCR assay for the detection of bovine Milk in foods.Food chemistry. Molecular sciences · 2026Article
- Molecular Investigation and Characterization of Enterocytozoon bieneusi, Coxiella burnetii and Dientamoeba fragilis in Raw Milk and Cheese: A First Report for Dientamoeba fragilis.Acta parasitologica · 2025Article
- Can qPCR on pooled sheep milk detect brucellosis as part of herd brucellosis control and eradication programs?BMC veterinary research · 2025Article
- A Rapid and Inexpensive PCR Test for Mastitis Diagnosis Based on NGS Data.Pathogens (Basel, Switzerland) · 2024Article
- Validation ofAnimals : an open access journal from MDPI · 2024Article
- Milk somatic cell DNA isolation and characterization of κ-casein gene in Halari donkey milk.Heliyon · 2024Article
- Beneficial probiotic bacteria prevalence in different lactating dromedary camel milk of Saudi Arabia.Saudi journal of biological sciences · 2024Article
- High resolution melting is a useful tool to detect animal species sources of various milk types.Journal of food science and technology · 2023Article
- Development of a duplex qPCR assay with locked nucleic acid probes for A, B and E kappa-casein variants detection.Scientific reports · 2022Article
- Occurrence and risk factors of brucellosis among domestic animals: an artificial neural network approach.Tropical animal health and production · 2022Article
- Impact of Bovine Lipocalin-2 Gene on the Antioxidant Activity of Milk from Polish Holstein-Friesian Cows.Animals : an open access journal from MDPI · 2019Article
- A fast and reliable polymerase chain reaction method based on short interspersed nuclear elements detection for the discrimination of buffalo, cattle, goat, and sheep species in dairy products.Asian-Australasian journal of animal sciences · 2019Article
- The influence of BoLA-DRB3 alleles on incidence of clinical mastitis, cystic ovary disease and milk traits in Holstein Friesian cattle.Molecular biology reports · 2018Article
- Molecular biological tools applied for identification of mastitis causing pathogens.International journal of veterinary science and medicine · 2017Review
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Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isolation of genomic DNA is one of the basic steps in many different molecular analyses. There are a few reports on methods of DNA isolation from milk, but many of them are time consuming and expensive, and require relatively large volumes of raw milk. In this study a rapid, sensitive, and efficient method of DNA extraction from milk somatic cells of various mammals (cattle, sheep, goats, horses) is presented. It was found that milk is a good source of genomic DNA, and to obtain a sufficient amount and quality of DNA, suitable for molecular analysis such as PCR, 10 mL of raw milk is sufficient. Thanks to this method, stress in animals can be reduced during collection of researched material. Therefore, this method could be widely used in molecular analyses.
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Registered trials
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