ArticleBiotechnology for biofuels2016
Engineering broad-spectrum digestion of polyuronides from an exolytic polysaccharide lyase.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 24 citations in OpenAlex.
- Dissecting a two-domain alginate lyase of family PL6 reveals a mechanistic basis for substrate specificity and enzyme activity.The Journal of biological chemistry · 2026Article
- Diatom heterotrophy on brown algal polysaccharides emerged through horizontal gene transfer, gene duplication, and neofunctionalization.PLoS biology · 2025Article
- Review
- A bifunctional endolytic alginate lyase with two different lyase catalytic domains fromFrontiers in microbiology · 2024Article
- A novel alginate lyase and its domain functions for the preparation of unsaturated monosaccharides.Applied microbiology and biotechnology · 2023Article
- Algicidal Bacteria: A Review of Current Knowledge and Applications to Control Harmful Algal Blooms.Frontiers in microbiology · 2022Review
- Bacterial alginate metabolism: an important pathway for bioconversion of brown algae.Biotechnology for biofuels · 2021Review
- Genomics Reveals the Metabolic Potential and Functions in the Redistribution of Dissolved Organic Matter in Marine Environments of the GenusMicroorganisms · 2020Article
- Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides.Biotechnology for biofuels · 2020Article
- Biochemical characteristics and synergistic effect of two novel alginate lyases fromBiotechnology for biofuels · 2019Article
- Ancient acquisition of "alginate utilization loci" by human gut microbiota.Scientific reports · 2018Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMacroalgae represents a promising source of fermentable carbohydrates for use in the production of energy efficient biofuel. The primary carbohydrate in brown algae is the uronic acid-containing alginate, whereas green algae contains a significant amount of glucuronan. A necessary step in the conversion of these polyuronides to bioethanol is saccharification, which can be achieved by enzymatic or chemical degradation.
resultsPolysaccharide lyases are a class of enzymes which cleave uronic acid-containing glycans via a β-elimination mechanism, acting both endo- and exolytically on their substrates. In the present work, we characterize a putative alginate lyase from Stenotrophomonas maltophilia K279a (Smlt2602) and describe a H208F mutant that, in addition to cleaving alginate-based substrates, displays significant, exolytic glucuronan activity.
conclusionsTo our knowledge this is the first polysaccharide lyase to act exolytically on glucuronan and is an attractive candidate for the broad-spectrum digestion of polyuronides into fermentable monomers.
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