ArticleMolecular biology of the cell2016
Divergent microtubule assembly rates after short- versus long-term loss of end-modulating kinesins.
Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- MCAK/Kif2C centromeric activity level tunes K-fiber turnover through distinct pathways.iScience · 2026Article
- KIF18A Maintains Kinetochore-Microtubule Attachments in CIN Cells by Limiting Microtubule Polymerization.bioRxiv : the preprint server for biology · 2026Article
- Article
- Production of CRISPR-Cas9 Transgenic Cell Lines for Knocksideways Studies.Current protocols · 2023Article
- The Proliferation of Glioblastoma Is Contributed to Kinesin Family Member 18A and Medical Data Analysis of GBM.Frontiers in genetics · 2022Article
- Chromosomally unstable tumor cells specifically require KIF18A for proliferation.Nature communications · 2021Article
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- Article
- Article
- Microtubule Growth Rates Are Sensitive to Global and Local Changes in Microtubule Plus-End Density.Current biology : CB · 2020Article
- Cell Biology: Social Distancing of Microtubule Ends Increases Their Assembly Rates.Current biology : CB · 2020Article
- Functional characterization of MCAK/Kif2C cancer mutations using high-throughput microscopic analysis.Molecular biology of the cell · 2020Article
- The quantification and regulation of microtubule dynamics in the mitotic spindle.Current opinion in cell biology · 2019Review
- GPR124 regulates microtubule assembly, mitotic progression, and glioblastoma cell proliferation.Glia · 2019Article
- TAPping into the treasures of tubulin using novel protein production methods.Essays in biochemistry · 2018Review
- The role of kinesin KIF18A in the invasion and metastasis of hepatocellular carcinoma.World journal of surgical oncology · 2018Article
- MCAK-mediated regulation of endothelial cell microtubule dynamics is mechanosensitive to myosin-II contractility.Molecular biology of the cell · 2017Article
- Spatial regulation of astral microtubule dynamics by Kif18B in PtK cells.Molecular biology of the cell · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Depletion of microtubule (MT) regulators can initiate stable alterations in MT assembly rates that affect chromosome instability and mitotic spindle function, but the manner by which cellular MT assembly rates can stably increase or decrease is not understood. To investigate this phenomenon, we measured the response of microtubule assembly to both rapid and long-term loss of MT regulators MCAK/Kif2C and Kif18A. Depletion of MCAK/Kif2C by siRNA stably decreases MT assembly rates in mitotic spindles, whereas depletion of Kif18A stably increases rates of assembly. Surprisingly, this is not phenocopied by rapid rapamycin-dependent relocalization of MCAK/Kif2C and Kif18A to the plasma membrane. Instead, this treatment yields opposite affects on MT assembly. Rapidly increased MT assembly rates are balanced by a decrease in nucleated microtubules, whereas nucleation appears to be maximal and limiting for decreased MT assembly rates and also for long-term treatments. We measured amplified tubulin synthesis during long-term depletion of MT regulators and hypothesize that this is the basis for different phenotypes arising from long-term versus rapid depletion of MT regulators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.