Evidence map›Paper›PMID 26912788›Full record

ArticleMolecular biology of the cell2016

Sorting nexin 27 couples PTHR trafficking to retromer for signal regulation in osteoblasts during bone growth.

Audrey S M Chan, Thomas Clairfeuille, Euphemie Landao-Bassonga, Genevieve Kinna, Pei Ying Ng, Li Shen Loo, Tak Sum Cheng, Minghao Zheng, Wanjin Hong, Rohan D Teasdale and 2 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.

0numbers the graph read from it
0cells of the map it votes in
33citing papers in PubMed
4.5field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

33 citing papers in PubMed, 53 citations in OpenAlex.

  1. Review
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  7. Review
  8. Endosomal parathyroid hormone receptor signaling.American journal of physiology. Cell physiology · 2022
    Review
  9. Article
  10. SNX27 suppresses SARS-CoV-2 infection by inhibiting viral lysosome/late endosome entry.Proceedings of the National Academy of Sciences of the United States of America · 2022
    Article
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  12. Article
  13. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Audrey S M ChanCellular Orthopaedic Laboratory, School of Surgery, University of Western Australia, Nedlands 6009, Australia.
Thomas ClairfeuilleInstitute for Molecular Bioscience, University of Queensland, St. Lucia 4072, Australia.
Euphemie Landao-BassongaCellular Orthopaedic Laboratory, School of Surgery, University of Western Australia, Nedlands 6009, Australia.
Genevieve KinnaInstitute for Molecular Bioscience, University of Queensland, St. Lucia 4072, Australia.
Pei Ying NgCellular Orthopaedic Laboratory, School of Surgery, University of Western Australia, Nedlands 6009, Australia.
Li Shen LooInstitute of Molecular and Cell Biology, A*STAR, Singapore 138673.
Tak Sum ChengCellular Orthopaedic Laboratory, School of Surgery, University of Western Australia, Nedlands 6009, Australia.
Minghao ZhengCellular Orthopaedic Laboratory, School of Surgery, University of Western Australia, Nedlands 6009, Australia.
Wanjin HongInstitute of Molecular and Cell Biology, A*STAR, Singapore 138673.
Rohan D TeasdaleInstitute for Molecular Bioscience, University of Queensland, St. Lucia 4072, Australia.
Brett M CollinsInstitute for Molecular Bioscience, University of Queensland, St. Lucia 4072, Australia nathan.pavlos@uwa.edu.au b.collins@imb.uq.edu.au.
Nathan J PavlosCellular Orthopaedic Laboratory, School of Surgery, University of Western Australia, Nedlands 6009, Australia nathan.pavlos@uwa.edu.au b.collins@imb.uq.edu.au.
University of Western Australia · AUUniversity of Queensland · AUInstitute of Molecular and Cell Biology · SG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The parathyroid hormone 1 receptor (PTHR) is central to the process of bone formation and remodeling. PTHR signaling requires receptor internalization into endosomes, which is then terminated by recycling or degradation. Here we show that sorting nexin 27 (SNX27) functions as an adaptor that couples PTHR to the retromer trafficking complex. SNX27 binds directly to the C-terminal PDZ-binding motif of PTHR, wiring it to retromer for endosomal sorting. The structure of SNX27 bound to the PTHR motif reveals a high-affinity interface involving conserved electrostatic interactions. Mechanistically, depletion of SNX27 or retromer augments intracellular PTHR signaling in endosomes. Osteoblasts genetically lacking SNX27 show similar disruptions in PTHR signaling and greatly reduced capacity for bone mineralization, contributing to profound skeletal deficits in SNX27-knockout mice. Taken together, our data support a critical role for SNX27-retromer mediated transport of PTHR in normal bone development.

Indexed as

AnimalsBone DevelopmentBone RemodelingCalcification, PhysiologicEndosomesHEK293 CellsHumansMice, KnockoutMultiprotein ComplexesOsteoblastsParathyroid HormonePDZ DomainsProtein TransportReceptor, Parathyroid Hormone, Type 1Signal TransductionSorting NexinsMultiprotein ComplexesParathyroid HormoneReceptor, Parathyroid Hormone, Type 1Snx27 protein, mouseSorting Nexins

Identifiers

PMID26912788
PMCPMC4831889
OpenAlexW2307384627

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.