ArticleMolecular & cellular proteomics : MCP2016
In Vivo Interaction Proteomics in Caenorhabditis elegans Embryos Provides New Insights into P Granule Dynamics.
Article in Molecular & cellular proteomics : MCP, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Germ granule components regulate endosomal switching during the oocyte-to-embryo transition in Caenorhabditis elegans.Communications biology · 2026Article
- Condensate-forming eIF4ET ensures adequate levels of meiotic proteins to support oocyte storage.Life science alliance · 2025Article
- Analysis ofComputational and structural biotechnology journal · 2024Article
- The Caenorhabditis elegans TDRD5/7-like protein, LOTR-1, interacts with the helicase ZNFX-1 to balance epigenetic signals in the germline.PLoS genetics · 2022Article
- Review
- Multiple roles of endocytosis and autophagy in intracellular remodeling during oocyte-to-embryo transition.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2022Review
- Regulation of biomolecular condensates by interfacial protein clusters.Science (New York, N.Y.) · 2021Article
- Article
- Microfluidics for Peptidomics, Proteomics, and Cell Analysis.Nanomaterials (Basel, Switzerland) · 2021Review
- Murine Cytomegalovirus M25 Proteins Sequester the Tumor Suppressor Protein p53 in Nuclear Accumulations.Journal of virology · 2020Article
- Germ Granules Govern Small RNA Inheritance.Current biology : CB · 2019Article
- Miniaturised interaction proteomics on a microfluidic platform with ultra-low input requirements.Nature communications · 2019Article
- Article
- The P Granules of C. elegans: A Genetic Model for the Study of RNA-Protein Condensates.Journal of molecular biology · 2018Review
- Evolutionary plasticity in the innate immune function of Akirin.PLoS genetics · 2018Article
- Polo-like Kinase Couples Cytoplasmic Protein Gradients in the C. elegans Zygote.Current biology : CB · 2018Article
- A continuum of mRNP complexes in embryonic microRNA-mediated silencing.Nucleic acids research · 2017Article
- Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 4 countries.
Funding
Abstract
Studying protein interactions in whole organisms is fundamental to understanding development. Here, we combine in vivo expressed GFP-tagged proteins with quantitative proteomics to identify protein-protein interactions of selected key proteins involved in early C. elegans embryogenesis. Co-affinity purification of interaction partners for eight bait proteins resulted in a pilot in vivo interaction map of proteins with a focus on early development. Our network reflects known biology and is highly enriched in functionally relevant interactions. To demonstrate the utility of the map, we looked for new regulators of P granule dynamics and found that GEI-12, a novel binding partner of the DYRK family kinase MBK-2, is a key regulator of P granule formation and germline maintenance. Our data corroborate a recently proposed model in which the phosphorylation state of GEI-12 controls P granule dynamics. In addition, we find that GEI-12 also induces granule formation in mammalian cells, suggesting a common regulatory mechanism in worms and humans. Our results show that in vivo interaction proteomics provides unique insights into animal development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.