ArticleScientific reports2016
CRISPR/Cas9-mediated GJA8 knockout in rabbits recapitulates human congenital cataracts.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 67 citations in OpenAlex.
- Through theGenes · 2024Review
- Genome-edited rabbits: Unleashing the potential of a promising experimental animal model across diverse diseases.Zoological research · 2024Review
- Characterization of a Novel Gja8 (Cx50) Mutation in a New Cataract Rat Model.Investigative ophthalmology & visual science · 2023Article
- The application and progression of CRISPR/Cas9 technology in ophthalmological diseases.Eye (London, England) · 2023Review
- New advances in CRISPR/Cas-mediated precise gene-editing techniques.Disease models & mechanisms · 2023Review
- Gene Therapeutic Delivery to the Salivary Glands.Advances in experimental medicine and biology · 2023Article
- Editorial: Applying large animals for developmental study and disease modeling.Frontiers in cell and developmental biology · 2023Article
- Highly efficient A-to-G base editing by ABE8.17 in rabbits.Molecular therapy. Nucleic acids · 2022Article
- A Novel Anti-Cancer Therapy: CRISPR/Cas9 Gene Editing.Frontiers in pharmacology · 2022Review
- No apparent p53 activation in CRISPR-engineered gene-edited rabbits.Journal of cellular and molecular medicine · 2021Article
- The role of gap junctions in cell death and neuromodulation in the retina.Neural regeneration research · 2021Review
- Toward precise CRISPR DNA fragment editing and predictable 3D genome engineering.Journal of molecular cell biology · 2021Review
- CRISPR-Cas9: A Preclinical and Clinical Perspective for the Treatment of Human Diseases.Molecular therapy : the journal of the American Society of Gene Therapy · 2021Review
- Gene Editing in Rabbits: Unique Opportunities for Translational Biomedical Research.Frontiers in genetics · 2021Review
- Genetically Modified Rabbits for Cardiovascular Research.Frontiers in genetics · 2021Review
- CRISPR Start-Loss: A Novel and Practical Alternative for Gene Silencing through Base-Editing-Induced Start Codon Mutations.Molecular therapy. Nucleic acids · 2020Article
- TheSaudi journal of biological sciences · 2020Article
- Genome engineering technologies in rabbits.Journal of biomedical research · 2020Article
- Connexin43 and connexin50 channels exhibit different permeability to the second messenger inositol triphosphate.Scientific reports · 2020Article
- Modeling ocular lens disease in Xenopus.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cataracts are the leading cause of vision loss in the world, although surgical treatment can restore vision in cataract patients. Until now, there have been no adequate animal models for in vivo studies of artificial lens safety and drug interactions. Genetic studies have demonstrated that GJA8 is involved in maintaining lens opacity and proper lens development. In this study, a cataract model with GJA8 gene knockout was developed via co-injection of Cas9/sgRNA mRNA into rabbit zygotes. Our results showed that gene mutation efficiency in the GJA8 locus reached 98.7% in embryos and 100% in pups, demonstrating that the Cas9/sgRNA system is a highly efficient tool for gene editing in rabbits. In agreement with other studies, our genetic and histology results showed that impaired GJA8 function caused microphthalmia, small lens size and cataracts. In summary, our novel rabbit model of cataracts will be an important drug-screening tool for cataract prevention and treatment.
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