ArticleJournal of virological methods2016
Artificial microRNA-derived resistance to Cassava brown streak disease.
Article in Journal of virological methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 65 citations in OpenAlex.
- miR398-Dirigent Module Negatively Regulates Cassava Resistance to Cassava Common Mosaic Virus by Affecting the Cell Wall Lignification and Thickness.Molecular plant pathology · 2025Article
- RNA silencing: the future potential strategy for engineering virus resistance in plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- The Emerging Applications of Artificial MicroRNA-Mediated Gene Silencing in Plant Biotechnology.Non-coding RNA · 2025Review
- Promising Biotechnological Applications of the Artificial Derivatives Designed and Constructed from Plant microRNA Genes.Plants (Basel, Switzerland) · 2025Review
- Research Progress on miRNAs and Artificial miRNAs in Insect and Disease Resistance and Breeding in Plants.Genes · 2024Review
- Advances in understanding plant-pathogen interactions: insights from tomato as a model system.Virusdisease · 2024Review
- Advances in the Study of the Transcriptional Regulation Mechanism of Plant miRNAs.Life (Basel, Switzerland) · 2023Review
- Artificial microRNA guide strand selection from duplexes with no mismatches shows a purine-rich preference for virus- and non-virus-based expression vectors in plants.Plant biotechnology journal · 2022Article
- Small RNA-based plant protection against diseases.Frontiers in plant science · 2022Review
- MicroRNAs: Potential Targets for Developing Stress-Tolerant Crops.Life (Basel, Switzerland) · 2021Review
- Silencing AC1 of Tomato leaf curl virus using artificial microRNA confers resistance to leaf curl disease in transgenic tomato.Plant cell reports · 2020Article
- Artificial Small RNA-Based Silencing Tools for Antiviral Resistance in Plants.Plants (Basel, Switzerland) · 2020Review
- Plant miRNA Cross-Kingdom Transfer Targeting Parasitic and Mutualistic Organisms as a Tool to Advance Modern Agriculture.Frontiers in plant science · 2020Review
- Engineering Disease-Resistant Cassava.Cold Spring Harbor perspectives in biology · 2019Review
- Artificial microRNA-mediated resistance to cucumber green mottle mosaic virus in Nicotiana benthamiana.Planta · 2019Article
- MicroRNAs and new biotechnological tools for its modulation and improving stress tolerance in plants.Plant biotechnology journal · 2019Review
- Review
- PAmiRDB: A web resource for plant miRNAs targeting viruses.Scientific reports · 2019Article
- Nanotechnology based approaches for detection and delivery of microRNA in healthcare and crop protection.Journal of nanobiotechnology · 2018Review
- Plant Responses to Pathogen Attack: Small RNAs in Focus.International journal of molecular sciences · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Artificial miRNAs (amiRNA) were generated targeting conserved sequences within the genomes of the two causal agents of Cassava brown streak disease (CBSD): Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV). Transient expression studies on ten amiRNAs targeting 21nt conserved sequences of P1(CBSV and UCBSV), P3(CBSV and UCBSV), CI(UCBSV), NIb(CBSV and UCBSV), CP(UCBSV) and the un-translated region (3'-UTR) were tested in Nicotiana benthamiana. Four out of the ten amiRNAs expressed the corresponding amiRNA at high levels. Transgenic N. benthamiana plants were developed for the four amiRNAs targeting the P1 and NIb genes of CBSV and the P1 and CP genes of UCBSV and shown to accumulate miRNA products. Transgenic plants challenged with CBSV and UCBSV isolates showed resistance levels that ranged between ∼20-60% against CBSV and UCBSV and correlated with expression levels of the transgenically derived miRNAs. MicroRNAs targeting P1 and NIb of CBSV showed protection against CBSV and UCBSV, while amiRNAs targeting the P1 and CP of UCBSV showed protection against UCBSV but were less efficient against CBSV. These results indicate a potential application of amiRNAs for engineering resistance to CBSD-causing viruses in cassava.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.