ArticleScientific reports2016
Near Infrared Fluorescence (NIRF) Molecular Imaging of Oxidized LDL with an Autoantibody in Experimental Atherosclerosis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Preclinical techniques to investigate exercise training in vascular pathophysiology.American journal of physiology. Heart and circulatory physiology · 2021Pooled it
- Optical theranostics in ischemic heart disease: from molecular insights to clinical translation.Theranostics · 2025Review
- Atherosclerotic cardiovascular disease in aging and the role of advanced cardiovascular imaging.NPJ cardiovascular health · 2024Review
- Invasive electrochemical impedance spectroscopy with phase delay for experimental atherosclerosis phenotyping.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2024Article
- Machine learning-directed electrical impedance tomography to predict metabolically vulnerable plaques.Bioengineering & translational medicine · 2024Article
- Nanomedicine for Diagnosis and Treatment of Atherosclerosis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Review
- Characteristics and evaluation of atherosclerotic plaques: an overview of state-of-the-art techniques.Frontiers in neurology · 2023Review
- Chemical and molecular tools to probe biological sex differences at multiple length scales.Journal of materials chemistry. B · 2022Review
- Intravascular Fluorescence Molecular Imaging of Atherosclerosis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Novel Immunoassay for Malondialdehyde-Conjugated Low-Density Lipoprotein Measures Dynamic Changes in the Blood of Patients Undergoing Coronary Artery Bypass Graft Surgery.Antioxidants (Basel, Switzerland) · 2021Article
- Coronary Magnetic Resonance Angiography in Chronic Coronary Syndromes.Frontiers in cardiovascular medicine · 2021Review
- Intravascular Molecular Imaging: Near-Infrared Fluorescence as a New Frontier.Frontiers in cardiovascular medicine · 2020Review
- Cardiovascular optoacoustics: From mice to men - A review.Photoacoustics · 2019Review
- In Vivo Near-Infrared Fluorescence Imaging of Atherosclerosis Using Local Delivery of Novel Targeted Molecular Probes.Scientific reports · 2019Article
- A Novel iRFP-Incorporated in vivo Murine Atherosclerosis Imaging System.Scientific reports · 2018Article
- Molecular imaging of atherosclerosis: spotlight on Raman spectroscopy and surface-enhanced Raman scattering.Heart (British Cardiac Society) · 2018Review
- PET Molecular Targets and Near-Infrared Fluorescence Imaging of Atherosclerosis.Current cardiology reports · 2018Review
- PET/MR Imaging of Malondialdehyde-Acetaldehyde Epitopes With a Human Antibody Detects Clinically Relevant Atherothrombosis.Journal of the American College of Cardiology · 2018Article
- Molecular imaging of MMP activity discriminates unstable from stable plaque phenotypes in shear-stress induced murine atherosclerosis.PloS one · 2018Article
- Molecular Imaging of Atherosclerosis: A Clinical Focus.Current cardiovascular imaging reports · 2017Article
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Authors and funding
15 authors at 5 institutions in 2 countries.
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Abstract
We aimed to develop a quantitative antibody-based near infrared fluorescence (NIRF) approach for the imaging of oxidized LDL in atherosclerosis. LO1, a well- characterized monoclonal autoantibody that reacts with malondialdehyde-conjugated LDL, was labeled with a NIRF dye to yield LO1-750. LO1-750 specifically identified necrotic core in ex vivo human coronary lesions. Injection of LO1-750 into high fat (HF) fed atherosclerotic Ldlr(-/-) mice led to specific focal localization within the aortic arch and its branches, as detected by fluorescence molecular tomography (FMT) combined with micro-computed tomography (CT). Ex vivo confocal microscopy confirmed LO1-750 subendothelial localization of LO1-750 at sites of atherosclerosis, in the vicinity of macrophages. When compared with a NIRF reporter of MMP activity (MMPSense-645-FAST), both probes produced statistically significant increases in NIRF signal in the Ldlr(-/-) model in relation to duration of HF diet. Upon withdrawing the HF diet, the reduction in oxLDL accumulation, as demonstrated with LO1-750, was less marked than the effect seen on MMP activity. In the rabbit, in vivo injected LO1-750 localization was successfully imaged ex vivo in aortic lesions with a customised intra-arterial NIRF detection catheter. A partially humanized chimeric LO1-Fab-Cys localized similarly to the parent antibody in murine atheroma showing promise for future translation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.