ArticleBMC veterinary research2016
Evaluation of different nested PCRs for detection of Anaplasma phagocytophilum in ruminants and ticks.
Article in BMC veterinary research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 31 citations in OpenAlex.
- Molecular Detection and Phylogenetic Analysis ofVeterinary sciences · 2025Article
- Tick species, tick-borne pathogen distribution and risk factor analysis in border areas of China, Russia and North Korea.Frontiers in veterinary science · 2025Article
- Genotyping of ticks: first molecular report ofFrontiers in cellular and infection microbiology · 2024Article
- Molecular Detection ofPathogens (Basel, Switzerland) · 2023Article
- Molecular identification ofIranian journal of microbiology · 2023Article
- Quantification of Acute and Chronic Theileriosis in Sheep by Quantitative PCR (qPCR).Archives of Razi Institute · 2023Article
- Molecular Detection ofMicroorganisms · 2022Article
- Molecular evidence of Anaplasma phagocytophilum in olive baboons and vervet monkeys in Kenya.BMC veterinary research · 2021Article
- Article
- Molecular Detection and Phylogeny ofAnimals : an open access journal from MDPI · 2021Article
- Molecular prevalence ofOpen veterinary journal · 2019Article
- Mutational analysis of gene function in the Anaplasmataceae: Challenges and perspectives.Ticks and tick-borne diseases · 2019Article
- Detecting and characterizing mixed infections with genetic variants of Anaplasma phagocytophilum in roe deer (Capreolus capreolus) by developing an ankA cluster-specific nested PCR.Parasites & vectors · 2017Article
- Molecular Detection ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2016Article
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAnaplasma phagocytophilum is a causative agent of granulocytic anaplasmosis in mammals, which has a broad geographical distribution and a high degree of clinical diversity. Currently, numerous PCR assays have been developed and used for the detection of A. phagocytophilum in various specimens. However, their performance varies. The aim of this study was to evaluate the performance of five nested PCR assays by detection of 363 ruminant and tick samples, and to select the most appropriate methods for the sensitive detection of A. phagocytophilum in environmental or clinical samples.
resultsPositive PCR results for A. phagocytophilum were obtained in 75 (20.7%), 42 (11.6%) and 19 (5.2%) specimens with primer sets EC (EC9/EC12a and SSAP2f/SSAP2r), EE (EE1/EE2 and EE3/EE4) and ge (ge3a/ge10r, ge9f/ge2), respectively. The amplification of template DNA with the primer set MSP (MAP4AP5/MSP4AP3, msp4f/msp4r) could not be obtained in both ruminants and ticks, and a low specificity of the EL primers [EL(569)F/EL(1193)R, EL(569)F/EL(1142)R] in tick samples was observed. Our results revealed that the nested PCR with primer set EC complementary to the 16S rRNA gene was the most sensitive assay for detection of A. phagocytophilum in ruminant and tick specimens. A. phagocytophilum was detected in 47 (35.1%) sheep, 12 (10.4%) cattle, and 17 (14.9%) ticks. Two A. phagocytophilum genotypes were identified, that varied between sheep and cattle in sample collection sites.
conclusionsThis report provides more valuable information for the diagnosis and management of granulocytic anaplasmosis in China.
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