ArticleScientific reports2016
Evaluation of Parameters for High Efficiency Transformation of Acinetobacter baumannii.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- PASIV: A Pooled Approach-Based Workflow to Overcome Toxicity-Induced Design of Experiments Failures and Inefficiencies.ACS synthetic biology · 2022Article
- Crc Regulates Succinate-Mediated Repression of Mineral Phosphate Solubilization inFrontiers in microbiology · 2021Article
- Atorvastatin does not display an antimicrobial activity on its own nor potentiates the activity of other antibiotics againstAccess microbiology · 2021Article
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- The H-NS Regulator Plays a Role in the Stress Induced by Carbapenemase Expression in Acinetobacter baumannii.mSphere · 2020Article
- Design of Experiments As a Tool for Optimization in Recombinant Protein Biotechnology: From Constructs to Crystals.Molecular biotechnology · 2019Review
- Intercellular Transfer of Chromosomal Antimicrobial Resistance Genes between Acinetobacter baumannii Strains Mediated by Prophages.Antimicrobial agents and chemotherapy · 2019Article
- Next Generation of TnApplied and environmental microbiology · 2019Article
- Development of a Transformation Method for Metschnikowia borealis and other CUG-Serine Yeasts.Genes · 2019Article
- Non-invasive nanosecond electroporation for biocontrol of surface infections: an in vivo study.Scientific reports · 2018Article
- Antibiotic Combinations That Enable One-Step, Targeted Mutagenesis of Chromosomal Genes.ACS infectious diseases · 2018Article
- New Shuttle Vectors for Gene Cloning and Expression in Multidrug-Resistant Acinetobacter Species.Antimicrobial agents and chemotherapy · 2018Article
- Antimicrobial Activity of Gallium Compounds on ESKAPE Pathogens.Frontiers in cellular and infection microbiology · 2018Article
- Membrane permeabilization of mammalian cells using bursts of high magnetic field pulses.PeerJ · 2017Article
- The Genetic Analysis of an Acinetobacter johnsonii Clinical Strain Evidenced the Presence of Horizontal Genetic Transfer.PloS one · 2016Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acinetobacter baumannii is an emerging, nosocomial pathogen that is poorly characterized due to a paucity of genetic tools and methods. While whole genome sequence data from several epidemic and environmental strains have recently become available, the functional characterization of genes is significantly lagging. Efficient transformation is one of the first steps to develop molecular tools that can be used to address these shortcomings. Here we report parameters allowing high efficiency transformation of A. baumannii. Using a multi-factorial experimental design we found that growth phase, voltage, and resistance all significantly contribute to transformation efficiency. The highest efficiency (4.3 × 10(8) Transformants/μg DNA) was obtained at the stationary growth phase of the bacterium (OD 6.0) using 25 ng of plasmid DNA under 100 Ohms resistance and 1.7 kV/cm voltage. The optimized electroporation parameters reported here provide a useful tool for genetic manipulation of A. baumannii.
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