ArticleOpen biology2016
Insights from the reconstitution of the divergent outer kinetochore of Drosophila melanogaster.
Article in Open biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 37 citations in OpenAlex.
- Retention of a single Cenp-C gene in different syntenic locations in the montium group of Drosophila species.Heredity · 2026Article
- Retention of a singlebioRxiv : the preprint server for biology · 2025Article
- Functional framework of the kinetochore and spindle assembly checkpoint in Arabidopsis.Plant physiology · 2025Article
- Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation inMolecular biology of the cell · 2024Article
- Coregulation of NDC80 Complex Subunits Determines the Fidelity of the Spindle-Assembly Checkpoint and Mitosis.Molecular cancer research : MCR · 2024Article
- Meiosis-specific functions of kinetochore protein SPC105R required for chromosome segregation inbioRxiv : the preprint server for biology · 2024Article
- Centromere structure and function: lessons from Drosophila.Genetics · 2023Article
- A dynamic population of prophase CENP-C is required for meiotic chromosome segregation.PLoS genetics · 2023Article
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- Bridgin connects the outer kinetochore to centromeric chromatin.Nature communications · 2021Article
- Meiotic CENP-C is a shepherd: bridging the space between the centromere and the kinetochore in time and space.Essays in biochemistry · 2020Review
- Cyclin B1 scaffolds MAD1 at the kinetochore corona to activate the mitotic checkpoint.The EMBO journal · 2020Article
- Evolutionary Repair Experiments as a Window to the Molecular Diversity of Life.Current biology : CB · 2020Review
- Article
- RNAi-mediated depletion of the NSL complex subunits leads to abnormal chromosome segregation and defective centrosome duplication in Drosophila mitosis.PLoS genetics · 2019Article
- Where is the right path heading from the centromere to spindle microtubules?Cell cycle (Georgetown, Tex.) · 2019Review
- The Kinetochore Protein Spc105, a Novel Interaction Partner of LaeA, Regulates Development and Secondary Metabolism inFrontiers in microbiology · 2019Article
- Article
- Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80.Molecular cell · 2018Article
- Concurrent Duplication of Drosophila Cid and Cenp-C Genes Resulted in Accelerated Evolution and Male Germline-Biased Expression of the New Copies.Journal of molecular evolution · 2018Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate chromosome segregation during mitosis and meiosis is crucial for cellular and organismal viability. Kinetochores connect chromosomes with spindle microtubules and are essential for chromosome segregation. These large protein scaffolds emerge from the centromere, a specialized region of the chromosome enriched with the histone H3 variant CENP-A. In most eukaryotes, the kinetochore core consists of the centromere-proximal constitutive centromere-associated network (CCAN), which binds CENP-A and contains 16 subunits, and of the centromere-distal Knl1 complex, Mis12 complex, Ndc80 complex (KMN) network, which binds microtubules and contains 10 subunits. In the fruitfly, Drosophila melanogaster, the kinetochore underwent remarkable simplifications. All CCAN subunits, with the exception of centromeric protein C (CENP-C), and two KMN subunits, Dsn1 and Zwint, cannot be identified in this organism. In addition, two paralogues of the KMN subunit Nnf1 (Nnf1a and Nnf1b) are present. Finally, the Spc105R subunit, homologous to human Knl1/CASC5, underwent considerable sequence changes in comparison with other organisms. We combined biochemical reconstitution with biophysical and structural methods to investigate how these changes reflect on the organization of the Drosophila KMN network. We demonstrate that the Nnf1a and Nnf1b paralogues are subunits of distinct complexes, both of which interact directly with Spc105R and with CENP-C, for the latter of which we identify a binding site on the Mis12 subunit. Our studies shed light on the structural and functional organization of a highly divergent kinetochore particle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.