ArticleOpen biology2016
Network of protein interactions within the Drosophila inner kinetochore.
Article in Open biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Retention of a single Cenp-C gene in different syntenic locations in the montium group of Drosophila species.Heredity · 2026Article
- Retention of a singlebioRxiv : the preprint server for biology · 2025Article
- CENP-C-targeted PLK-1 regulates kinetochore function in C. elegans embryos.Journal of cell science · 2024Article
- A dynamic population of prophase CENP-C is required for meiotic chromosome segregation.PLoS genetics · 2023Article
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- Bridgin connects the outer kinetochore to centromeric chromatin.Nature communications · 2021Article
- Biochemical evidence for diverse strategies in the inner kinetochore.Open biology · 2020Review
- Similar but Not Identical-Binding Properties of LSU (Response to Low Sulfur) Proteins FromFrontiers in plant science · 2020Article
- A role of the Trx-G complex in Cid/CENP-A deposition at Drosophila melanogaster centromeres.Chromosoma · 2019Article
- Where is the right path heading from the centromere to spindle microtubules?Cell cycle (Georgetown, Tex.) · 2019Review
- Reconstitution of a 26-Subunit Human Kinetochore Reveals Cooperative Microtubule Binding by CENP-OPQUR and NDC80.Molecular cell · 2018Article
- Phosphorylation of CENP-C by Aurora B facilitates kinetochore attachment error correction in mitosis.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- A Molecular View of Kinetochore Assembly and Function.Biology · 2017Review
- Structural Dynamics of the Vimentin Coiled-coil Contact Regions Involved in Filament Assembly as Revealed by Hydrogen-Deuterium Exchange.The Journal of biological chemistry · 2016Article
- Article
- The unconventional kinetoplastid kinetochore: from discovery toward functional understanding.Biochemical Society transactions · 2016Review
- Getting to the heart of an unusual kinetochore.Open biology · 2016Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The kinetochore provides a physical connection between microtubules and the centromeric regions of chromosomes that is critical for their equitable segregation. The trimeric Mis12 sub-complex of the Drosophila kinetochore binds to the mitotic centromere using CENP-C as a platform. However, knowledge of the precise connections between Mis12 complex components and CENP-C has remained elusive despite the fundamental importance of this part of the cell division machinery. Here, we employ hydrogen-deuterium exchange coupled with mass spectrometry to reveal that Mis12 and Nnf1 form a dimer maintained by interacting coiled-coil (CC) domains within the carboxy-terminal parts of both proteins. Adjacent to these interacting CCs is a carboxy-terminal domain that also interacts with Nsl1. The amino-terminal parts of Mis12 and Nnf1 form a CENP-C-binding surface, which docks the complex and thus the entire kinetochore to mitotic centromeres. Mutational analysis confirms these precise interactions are critical for both structure and function of the complex. Thus, we conclude the organization of the Mis12-Nnf1 dimer confers upon the Mis12 complex a bipolar, elongated structure that is critical for kinetochore function.
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Registered trials
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