ArticleGlycobiology2016
Combining 3D structure with glycan array data provides insight into the origin of glycan specificity.
Article in Glycobiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 47 citations in OpenAlex.
- GlyContact analyzes glycan 3D structures at scale.Nature communications · 2025Article
- Stinging Nettle (Plants (Basel, Switzerland) · 2025Review
- Examining the Influenza A Virus Sialic Acid Binding Preference Predictions of a Sequence-Based Convolutional Neural Network.Influenza and other respiratory viruses · 2024Article
- Glycosaminoglycan microarrays for studying glycosaminoglycan-protein systems.Carbohydrate polymers · 2024Review
- Lectin Fingerprinting Distinguishes Antibody Neutralization in SARS-CoV-2.ACS central science · 2023Article
- Proximity labeling technologies to illuminate glycan-protein interactions.Current opinion in chemical biology · 2023Review
- Simplifying the detection and monitoring of protein glycosylation during in vitro glycoengineering.Scientific reports · 2023Article
- Modeling of Protein Complexes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A Useful Guide to Lectin Binding: Machine-Learning Directed Annotation of 57 Unique Lectin Specificities.ACS chemical biology · 2022Article
- GlyNet: a multi-task neural network for predicting protein-glycan interactions.Chemical science · 2022Article
- Seeing the forest through the trees: characterizing the glycoproteome.Trends in biochemical sciences · 2022Review
- Comprehensive analysis of lectin-glycan interactions reveals determinants of lectin specificity.PLoS computational biology · 2021Article
- Combined Analysis of Multiple Glycan-Array Datasets: New Explorations of Protein-Glycan Interactions.Analytical chemistry · 2021Article
- Calculating Glycoprotein Similarities From Mass Spectrometric Data.Molecular & cellular proteomics : MCP · 2021Review
- Three-Dimensional Structures of Carbohydrates and Where to Find Them.International journal of molecular sciences · 2020Review
- Glycomics and glycoproteomics of viruses: Mass spectrometry applications and insights toward structure-function relationships.Mass spectrometry reviews · 2020Review
- Microarray analyses of closely related glycoforms reveal different accessibilities of glycan determinants on N-glycan branches.Glycobiology · 2020Article
- Advances in Tools to Determine the Glycan-Binding Specificities of Lectins and Antibodies.Molecular & cellular proteomics : MCP · 2020Review
- Tools for generating and analyzing glycan microarray data.Beilstein journal of organic chemistry · 2020Review
- Deciphering Protein Glycosylation by Computational Integration of On-chip Profiling, Glycan-array Data, and Mass Spectrometry.Molecular & cellular proteomics : MCP · 2019Article
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6 authors at 2 institutions in 1 country.
Funding
Abstract
Defining how a glycan-binding protein (GBP) specifically selects its cognate glycan from among the ensemble of glycans within the cellular glycome is an area of intense study. Powerful insight into recognition mechanisms can be gained from 3D structures of GBPs complexed to glycans; however, such structures remain difficult to obtain experimentally. Here an automated 3D structure generation technique, called computational carbohydrate grafting, is combined with the wealth of specificity information available from glycan array screening. Integration of the array data with modeling and crystallography allows generation of putative co-complex structures that can be objectively assessed and iteratively altered until a high level of agreement with experiment is achieved. Given an accurate model of the co-complexes, grafting is also able to discern which binding determinants are active when multiple potential determinants are present within a glycan. In some cases, induced fit in the protein or glycan was necessary to explain the observed specificity, while in other examples a revised definition of the minimal binding determinants was required. When applied to a collection of 10 GBP-glycan complexes, for which crystallographic and array data have been reported, grafting provided a structural rationalization for the binding specificity of >90% of 1223 arrayed glycans. A webtool that enables researchers to perform computational carbohydrate grafting is available at www.glycam.org/gr (accessed 03 March 2016).
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.