ArticleBiophysical journal2016
Single-Molecule Imaging in Living Drosophila Embryos with Reflected Light-Sheet Microscopy.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.Nano letters · 2026Article
- Versatile and Scalable Reflective Micromirrors for Single-Objective Light Sheet Microscopy.bioRxiv : the preprint server for biology · 2026Article
- Single-Molecule Localization Microscopy in vivo at the Drosophila Neuromuscular Junction.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Light-sheet microscopy enabled by a miniaturized plane illuminator.Biomedical optics express · 2025Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.Nature communications · 2024Article
- Whole-cell multi-target single-molecule super-resolution imaging in 3D with microfluidics and a single-objective tilted light sheet.bioRxiv : the preprint server for biology · 2024Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics.Npj imaging · 2024Review
- Enhancing obSTORM imaging performance with cubic spline PSF modeling.Biomedical optics express · 2023Article
- OptoRheo: Simultaneous in situ micro-mechanical sensing and imaging of live 3D biological systems.Communications biology · 2023Article
- Resolution doubling in light-sheet microscopy via oblique plane structured illumination.Nature methods · 2022Article
- Single-molecule tracking of Nodal and Lefty in live zebrafish embryos supports hindered diffusion model.Nature communications · 2022Article
- Single-Lens Light-Sheet Fluorescence Microscopy Based on Micro-Mirror Array.Laser & photonics reviews · 2022Article
- Rapid 3D-STORM imaging of diverse molecular targets in tissue.Cell reports methods · 2022Article
- Fast and parallel nanoscale three-dimensional tracking of heterogeneous mammalian chromatin dynamics.Molecular biology of the cell · 2022Article
- Analysis of intracellular protein dynamics in living zebrafish embryos using light-sheet fluorescence single-molecule microscopy.Biomedical optics express · 2021Article
- Light Sheet Illumination for 3D Single-Molecule Super-Resolution Imaging of Neuronal Synapses.Frontiers in synaptic neuroscience · 2021Review
- Oblique-plane single-molecule localization microscopy for tissues and small intact animals.Nature methods · 2019Article
- SMALL-LABS: Measuring Single-Molecule Intensity and Position in Obscuring Backgrounds.Biophysical journal · 2019Article
- Applications of Light-Sheet Microscopy in Microdevices.Frontiers in neuroanatomy · 2019Review
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In multicellular organisms, single-fluorophore imaging is obstructed by high background. To achieve a signal/noise ratio conducive to single-molecule imaging, we adapted reflected light-sheet microscopy (RLSM) to image highly opaque late-stage Drosophila embryos. Alignment steps were modified by means of commercially available microprisms attached to standard coverslips. We imaged a member of the septate-junction complex that was used to outline the three-dimensional epidermal structures of Drosophila embryos. Furthermore, we show freely diffusing single 10 kDa Dextran molecules conjugated to one to two Alexa647 dyes inside living embryos. We demonstrate that Dextran diffuses quickly (∼6.4 μm(2)/s) in free space and obeys directional movement within the epidermal tissue (∼0.1 μm(2)/s). Our single-particle-tracking results are supplemented by imaging the endosomal marker Rab5-GFP and by earlier reports on the spreading of morphogens and vesicles in multicellular organisms. The single-molecule results suggest that RLSM will be helpful in studying single molecules or complexes in multicellular organisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.