Evidence map›Paper›PMID 26910419›Full record

ArticleBiophysical journal2016

A Versatile High-Vacuum Cryo-transfer System for Cryo-microscopy and Analytics.

Sebastian Tacke, Vladislav Krzyzanek, Harald Nüsse, Roger Albert Wepf, Jürgen Klingauf, Rudolf Reichelt

Abstract read
In one paragraph

Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Cryogenic light microscopy of vitrified samples with angstrom precision.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  2. Article
  3. Review
  4. Article
  5. Recent advances and current trends in cryo-electron microscopy.Current opinion in structural biology · 2022
    Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Sebastian TackeInstitute of Medical Physics and Biophysics, University of Münster, Münster, Germany. Electronic address: s.tacke@uni-muenster.de.
Vladislav KrzyzanekInstitute of Scientific Instruments, The Czech Academy of Sciences, Brno, Czech Republic.
Harald NüsseInstitute of Medical Physics and Biophysics, University of Münster, Münster, Germany.
Roger Albert WepfScientific Center for Optical and Electron Microscopy, ETH Zürich, Zürich, Switzerland.
Jürgen KlingaufInstitute of Medical Physics and Biophysics, University of Münster, Münster, Germany.
Rudolf ReicheltInstitute of Medical Physics and Biophysics, University of Münster, Münster, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cryogenic microscopy methods have gained increasing popularity, as they offer an unaltered view on the architecture of biological specimens. As a prerequisite, samples must be handled under cryogenic conditions below their recrystallization temperature, and contamination during sample transfer and handling must be prevented. We present a high-vacuum cryo-transfer system that streamlines the entire handling of frozen-hydrated samples from the vitrification process to low temperature imaging for scanning transmission electron microscopy and transmission electron microscopy. A template for cryo-electron microscopy and multimodal cryo-imaging approaches with numerous sample transfer steps is presented.

Indexed as

VacuumArtifactsCold TemperatureCryoelectron MicroscopyIceTobacco Mosaic VirusIce

Identifiers

PMID26910419
PMCPMC4776042

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.