ArticleRoyal Society open science2016
A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding.
Article in Royal Society open science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Upscaling Genotyping by Amplicon Sequencing With GBAS-GUI.Molecular ecology resources · 2026Article
- Bridging the gap between legacy polymerase chain reaction-based microsatellite data with high-throughput sequencing data for conservation genomics.The Journal of heredity · 2026Article
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- First Assembly of a Draft Genome of the Critically Endangered Northern Muriqui (Ecology and evolution · 2025Article
- MhGeneS: An Analytical Pipeline to Allow for Robust Microhaplotype Genotyping.Molecular ecology resources · 2025Article
- Microsatellites reveal divergence in population genetic diversity, and structure of osyris lanceolata (santalaceae) in Uganda and Kenya.BMC ecology and evolution · 2023Article
- Rapid and easy construction of a simplified amplicon sequencing (simplified AmpSeq) library for marker-assisted selection.Scientific reports · 2023Article
- Article
- A baseline for the genetic stock identification of Atlantic herring,Royal Society open science · 2022Article
- Knowledge Gaps in Taxonomy, Ecology, Population Distribution Drivers and Genetic Diversity of African Sandalwood (Plants (Basel, Switzerland) · 2021Article
- Facilitating population genomics of non-model organisms through optimized experimental design for reduced representation sequencing.BMC genomics · 2021Article
- Dispersal ability, habitat characteristics, and sea-surface circulation shape population structure of Cingula trifasciata (Gastropoda: Rissoidae) in the remote Azores Archipelago.BMC ecology and evolution · 2021Article
- Revisiting traditional SSR based methodologies available for elephant genetic studies.Scientific reports · 2021Article
- Evidence for multiple introductions of an invasive wild bee species currently under rapid range expansion in Europe.BMC ecology and evolution · 2021Article
- A refined panel of 42 microsatellite loci to universally genotype catarrhine primates.Ecology and evolution · 2021Article
- Article
- MultiplexSSR: A pipeline for developing multiplex SSR-PCR assays from resequencing data.Ecology and evolution · 2020Article
- Molecular genetic diversity and differentiation of Nile tilapia (Oreochromis niloticus, L. 1758) in East African natural and stocked populations.BMC evolutionary biology · 2020Article
- Multiple and Extra-Pair Mating in a Pair-Living Hermaphrodite, the Intertidal LimpetIntegrative organismal biology (Oxford, England) · 2020Article
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This study examines the potential of next-generation sequencing based 'genotyping-by-sequencing' (GBS) of microsatellite loci for rapid and cost-effective genotyping in large-scale population genetic studies. The recovery of individual genotypes from large sequence pools was achieved by PCR-incorporated combinatorial barcoding using universal primers. Three experimental conditions were employed to explore the possibility of using this approach with existing and novel multiplex marker panels and weighted amplicon mixture. The GBS approach was validated against microsatellite data generated by capillary electrophoresis. GBS allows access to the underlying nucleotide sequences that can reveal homoplasy, even in large datasets and facilitates cross laboratory transfer. GBS of microsatellites, using individual combinatorial barcoding, is potentially faster and cheaper than current microsatellite approaches and offers better and more data.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.