ArticleBiomicrofluidics2016
Highly efficient and gentle trapping of single cells in large microfluidic arrays for time-lapse experiments.
Article in Biomicrofluidics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 26 citations in OpenAlex.
- Synthetic gene circuit evolution: Insights and opportunities at the mid-scale.Cell chemical biology · 2024Review
- Towards Small Scale: Overview and Applications of Microfluidics in Biotechnology.Molecular biotechnology · 2024Review
- Automated measurement of cell mechanical properties using an integrated dielectrophoretic microfluidic device.iScience · 2022Article
- Highly flexible elastomer microfluidic chip for single cell manipulation.Biomicrofluidics · 2022Article
- Nanovolcano microelectrode arrays: toward long-term on-demand registration of transmembrane action potentials by controlled electroporation.Microsystems & nanoengineering · 2020Article
- Efficient Low Shear Flow-based Trapping of Biological Entities.Scientific reports · 2019Article
- An integrated microfluidic platform for size-selective single-cell trapping of monocytes from blood.Biomicrofluidics · 2018Article
- Review
- On-chip cell labelling and washing by capture and release using microfluidic trap arrays.Biomicrofluidics · 2017Article
- Transport of biomolecules to binding partners displayed on the surface of microbeads arrayed in traps in a microfluidic cell.Biomicrofluidics · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The isolation of single biological cells and their further cultivation in dedicated arrayed chambers are key to the collection of statistically reliable temporal data in cell-based biological experiments. In this work, we present a hydrodynamic single cell trapping and culturing platform that facilitates cell observation and experimentation using standard bio-lab equipment. The proposed design leverages the stochastic position of the cells as they flow into the structured microfluidic channels, where hundreds of single cells are then arrayed in nanoliter chambers for simultaneous cell specific data collection. Numerical simulation tools are used to devise and implement a hydrodynamic cell trapping mechanism that is minimally detrimental to the cell cycle and retains high overall trapping efficiency (∼70%) with the capability of reaching high fill factors (>90%) in short loading times (1-4 min) in a 400-trap device. A Monte Carlo model is developed using the design parameters to estimate the system trapping efficiencies, which show strong agreement with the experimentally acquired data. As proof of concept, arrayed mammalian tissue cells (MIA PaCa-2) are cultured in the microfluidic chambers for two days without viability problems.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.