ArticleScientific reports2016
Exploring miniature insect brains using micro-CT scanning techniques.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- Article
- When a bigger brain is better: The case of bee olfactory learning.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Micro-computed tomography of termite gut anatomy: effects of staining regime on resolvability.PeerJ · 2026Article
- Optimizing Computed Tomography (CT) for Structurally Complex Organisms: A Case Study of Gastropod Shell Features.Ecology and evolution · 2025Article
- Article
- Sub-lethal imidacloprid exposure leads to presynaptic and postsynaptic alterations.Research square · 2025Article
- Differential performance of aqueous- and ethylic-Lugol's iodine stain to visualize anatomy in μCT-scanned vertebrates.Journal of anatomy · 2025Article
- Sexual dimorphism and morphological integration in the orchid bee brain.Scientific reports · 2025Article
- Comparing microCT Staining and Scanning Methodology for Brain Studies in Various Sizes of Spiders.The Journal of comparative neurology · 2025Article
- The SmARTR pipeline: A modular workflow for the cinematic rendering of 3D scientific imaging data.iScience · 2024Article
- Exploring the anatomy ofInternational journal for parasitology. Parasites and wildlife · 2024Article
- Anatomical changes of Tenebrio molitor and Tribolium castaneum during complete metamorphosis.Cell and tissue research · 2024Article
- Article
- Bioimaging marine crustacean brain: quantitative comparison of micro-CT preparations in an Alpheid snapping shrimp.Frontiers in neuroscience · 2024Article
- Article
- Multiple axes of visual system diversity in Ithomiini, an ecologically diverse tribe of mimetic butterflies.The Journal of experimental biology · 2023Article
- Natural variability in bee brain size and symmetry revealed by micro-CT imaging and deep learning.PLoS computational biology · 2023Article
- Novel Methodology for Localizing and Studying Insect Dorsal Rim Area Morphology in 2D and 3D.Insects · 2023Article
- Multiplexed neuropeptide mapping in ant brains integrating microtomography and three-dimensional mass spectrometry imaging.PNAS nexus · 2023Article
- Measuring compound eye optics with microscope and microCT images.Communications biology · 2023Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The capacity to explore soft tissue structures in detail is important in understanding animal physiology and how this determines features such as movement, behaviour and the impact of trauma on regular function. Here we use advances in micro-computed tomography (micro-CT) technology to explore the brain of an important insect pollinator and model organism, the bumblebee (Bombus terrestris). Here we present a method for accurate imaging and exploration of insect brains that keeps brain tissue free from trauma and in its natural stereo-geometry, and showcase our 3D reconstructions and analyses of 19 individual brains at high resolution. Development of this protocol allows relatively rapid and cost effective brain reconstructions, making it an accessible methodology to the wider scientific community. The protocol describes the necessary steps for sample preparation, tissue staining, micro-CT scanning and 3D reconstruction, followed by a method for image analysis using the freeware SPIERS. These image analysis methods describe how to virtually extract key composite structures from the insect brain, and we demonstrate the application and precision of this method by calculating structural volumes and investigating the allometric relationships between bumblebee brain structures.
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