ArticleScientific reports2016
Vitrified canine testicular cells allow the formation of spermatogonial stem cells and seminiferous tubules following their xenotransplantation into nude mice.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Germ cells are essential for testicular morphogenesis and functional reconstruction in a porcine xenograft model.Scientific reports · 2026Article
- Spermatogonial Stem Cells in Domestic Animals: Current Insights and Future Directions with a Focus on Dogs.Veterinary sciences · 2025Review
- Evaluation of Different Cryoprotectant Combinations in Testicular Vitrification in Dogs.Reproduction in domestic animals = Zuchthygiene · 2025Article
- Cryopreservation and culture strategies for testicular tissue and cells in small and large animals.Frontiers in veterinary science · 2025Review
- Optimizing Immature Testicular Tissue and Cell Transplantation Results: Comparing Transplantation Sites and Scaffolds.International journal of fertility & sterility · 2023Review
- Detection of spermatogonial stem cells in testicular tissue of dogs with chronic asymptomatic orchitis.Frontiers in veterinary science · 2023Article
- Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro.Development (Cambridge, England) · 2022Article
- Association of selected polymorphisms inInternational journal of reproductive biomedicine · 2022Article
- Article
- Canine and Feline Testicular Preservation.Animals : an open access journal from MDPI · 2022Review
- Current scenario and challenges ahead in application of spermatogonial stem cell technology in livestock.Journal of assisted reproduction and genetics · 2021Review
- Strategies for cryopreservation of testicular cells and tissues in cancer and genetic diseases.Cell and tissue research · 2021Review
- Canid Reproductive Biology: Norm and Unique Aspects in Strategies and Mechanisms.Animals : an open access journal from MDPI · 2021Review
- EGF, GDNF, and IGF-1 influence the proliferation and stemness of ovine spermatogonial stem cells in vitro.Journal of assisted reproduction and genetics · 2020Article
- Engineered reproductive tissues.Nature biomedical engineering · 2020Review
- Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.Oxidative medicine and cellular longevity · 2020Article
- CD14 is a unique membrane marker of porcine spermatogonial stem cells, regulating their differentiation.Scientific reports · 2019Article
- Xenotransplantation of canine spermatogonial stem cells (cSSCs) regulated by FSH promotes spermatogenesis in infertile mice.Stem cell research & therapy · 2019Article
- Tracing and Characterizing the Development of Transplanted Female Germline Stem Cells In Vivo.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Belgian Malinois (BM), one of the excellent military dog breeds in South Korea, is usually castrated before sexual maturation. Therefore, the transfer of their genetic features to the next generation is difficult. To overcome this, testicular cells from 4-month-old BMs were frozen. Testicular cells were thawed after 3 months and cultured in StemPro-34 medium. Spermatogonial stem cell (SSC) characteristics were determined by the transplantation of the cultured germ cell-derived colonies (GDCs) into empty testes, containing only several endogenous SSCs and Sertoli cells, of immunodeficient mice, 4 weeks after busulfan treatment. Following the implantation, the transplanted cells localized in the basement membrane of the seminiferous tubules, and ultimately colonized the recipient testes. Xenotransplantation of GDCs together with testicular somatic cells conjugated with extracellular matrix (ECM), led to the formation of de novo seminiferous tubules. These seminiferous tubules were mostly composed of Sertoli cells. Some germ cells were localized in the basement membrane of seminiferous tubules. This study revealed that BM-derived SSCs, obtained from the castrated testes, might be a valuable tool for the transfer of BM genetic features to the next generation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.