ArticleBiomedical microdevices2016
Polyethersulfone improves isothermal nucleic acid amplification compared to current paper-based diagnostics.
Article in Biomedical microdevices, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 68 citations in OpenAlex.
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- Extraction-Free Scalable Sample Preparation for the Rapid Detection of HIV in Blood Plasma.Analytical chemistry · 2026Article
- Paper-Based Microfluidic Devices: A Powerful Strategy for Rapid Detection.Micromachines · 2025Review
- Vibration mixing for enhanced paper-based recombinase polymerase amplification.Lab on a chip · 2024Article
- A Point-of-Care Nucleic Acid Quantification Method by Counting Light Spots Formed by LAMP Amplicons on a Paper Membrane.Biosensors · 2024Article
- Self-Diagnosis of SARS-CoV-2 from Saliva Samples at Home: Isothermal Amplification Enabled by Do-It-Yourself Portable Incubators and Laminated Poly-ethyl Sulfonate Membranes.Diagnostics (Basel, Switzerland) · 2024Article
- Promise and perils of paper-based point-of-care nucleic acid detection for endemic and pandemic pathogens.Lab on a chip · 2023Review
- Quantitative isothermal amplification on paper membranes using amplification nucleation site analysis.Lab on a chip · 2022Article
- Quantifying DNA damage on paper sensorsChemical science · 2022Article
- Strategies for Engineering Affordable Technologies for Point-of-Care Diagnostics of Infectious Diseases.Accounts of chemical research · 2021Review
- Minimally instrumented SHERLOCK (miSHERLOCK) for CRISPR-based point-of-care diagnosis of SARS-CoV-2 and emerging variants.Science advances · 2021Article
- Applications of electrowetting-on-dielectric (EWOD) technology for droplet digital PCR.Biomicrofluidics · 2020Review
- Current and Future Perspectives on Isothermal Nucleic Acid Amplification Technologies for Diagnosing Infections.Infection and drug resistance · 2020Review
- Microfluidic rapid and autonomous analytical device (microRAAD) to detect HIV from whole blood samples.Lab on a chip · 2019Article
- Integrated microfluidic systems with sample preparation and nucleic acid amplification.Lab on a chip · 2019Review
- Paper-based microfluidics for DNA diagnostics of malaria in low resource underserved rural communities.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Inorganic Complexes and Metal-Based Nanomaterials for Infectious Disease Diagnostics.Chemical reviews · 2019Review
- Development of a Membrane-Based Method for Isolation of Genomic DNA from Human Blood.Journal of biomolecular techniques : JBT · 2018Article
- Profile of the Alere i Influenza A & B assay: a pioneering molecular point-of-care test.Expert review of molecular diagnostics · 2018Article
- A paperfluidic platform to detect Neisseria gonorrhoeae in clinical samples.Biomedical microdevices · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Devices based on rapid, paper-based, isothermal nucleic acid amplification techniques have recently emerged with the potential to fill a growing need for highly sensitive point-of-care diagnostics throughout the world. As this field develops, such devices will require optimized materials that promote amplification and sample preparation. Herein, we systematically investigated isothermal nucleic acid amplification in materials currently used in rapid diagnostics (cellulose paper, glass fiber, and nitrocellulose) and two additional porous membranes with upstream sample preparation capabilities (polyethersulfone and polycarbonate). We compared amplification efficiency from four separate DNA and RNA targets (Bordetella pertussis, Chlamydia trachomatis, Neisseria gonorrhoeae, and Influenza A H1N1) within these materials using two different isothermal amplification schemes, helicase dependent amplification (tHDA) and loop-mediated isothermal amplification (LAMP), and traditional PCR. We found that the current paper-based diagnostic membranes inhibited nucleic acid amplification when compared to membrane-free controls; however, polyethersulfone allowed for efficient amplification in both LAMP and tHDA reactions. Further, observing the performance of traditional PCR amplification within these membranes was not predicative of their effects on in situ LAMP and tHDA. Polyethersulfone is a new material for paper-based nucleic acid amplification, yet provides an optimal support for rapid molecular diagnostics for point-of-care applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.