ArticleDevelopmental cell2016
Quantitative Multiscale Cell Imaging in Controlled 3D Microenvironments.
Article in Developmental cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 77 citations in OpenAlex.
- Universal consensus 3D segmentation of cells from 2D segmented stacks.Nature methods · 2025Article
- Direct Arp2/3-vinculin binding is required for pseudopod extension, but only on compliant substrates and in 3D.iScience · 2025Article
- Cell dynamics revealed by microscopy advances.Current opinion in cell biology · 2024Review
- Proteolysis-free amoeboid migration of melanoma cells through crowded environments via bleb-driven worrying.Developmental cell · 2024Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Exploring the Role of the Plant Actin Cytoskeleton: From Signaling to Cellular Functions.International journal of molecular sciences · 2023Review
- Cellular harmonics for the morphology-invariant analysis of molecular organization at the cell surface.Nature computational science · 2023Article
- Extended Depth of Focus Two-Photon Light-Sheet Microscopy for In Vivo Fluorescence Imaging of Large Multicellular Organisms at Cellular Resolution.International journal of molecular sciences · 2023Article
- High-resolution confocal and light-sheet imaging of collagen 3D network architecture in very large samples.iScience · 2023Article
- Surface-guided computing to analyze subcellular morphology and membrane-associated signals in 3D.bioRxiv : the preprint server for biology · 2023Article
- Surface-guided computing to analyze subcellular morphology and membrane-associated signals in 3D.ArXiv · 2023Article
- Article
- Isotropic imaging across spatial scales with axially swept light-sheet microscopy.Nature protocols · 2022Review
- Light-Sheet Scattering Microscopy to Visualize Long-Term Interactions Between Cells and Extracellular Matrix.Frontiers in immunology · 2022Article
- Article
- Interpretable deep learning uncovers cellular properties in label-free live cell images that are predictive of highly metastatic melanoma.Cell systems · 2021Article
- Wide field light-sheet microscopy with lens-axicon controlled two-photon Bessel beam illumination.Nature communications · 2021Article
- Systematic alteration of in vitro metabolic environments reveals empirical growth relationships in cancer cell phenotypes.Cell reports · 2021Article
- Bioimage Analysis and Cell Motility.Patterns (New York, N.Y.) · 2021Review
- Actin-Membrane Release Initiates Cell Protrusions.Developmental cell · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The microenvironment determines cell behavior, but the underlying molecular mechanisms are poorly understood because quantitative studies of cell signaling and behavior have been challenging due to insufficient spatial and/or temporal resolution and limitations on microenvironmental control. Here we introduce microenvironmental selective plane illumination microscopy (meSPIM) for imaging and quantification of intracellular signaling and submicrometer cellular structures as well as large-scale cell morphological and environmental features. We demonstrate the utility of this approach by showing that the mechanical properties of the microenvironment regulate the transition of melanoma cells from actin-driven protrusion to blebbing, and we present tools to quantify how cells manipulate individual collagen fibers. We leverage the nearly isotropic resolution of meSPIM to quantify the local concentration of actin and phosphatidylinositol 3-kinase signaling on the surfaces of cells deep within 3D collagen matrices and track the many small membrane protrusions that appear in these more physiologically relevant environments.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.