ArticlePloS one2016
Crystal Structure of Alcohol Oxidase from Pichia pastoris.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 62 citations in OpenAlex.
- Evolution of alcohol oxidase for improved methanol bioconversion and formaldehyde tolerance.Synthetic and systems biotechnology · 2027Article
- Metabolic engineering of Escherichia coli for methanol-assisted resveratrol biosynthesis.Bioprocess and biosystems engineering · 2026Article
- Cryo-EM Structures of Alcohol Oxidase Isozymes Reveal Structural Determinants of Cofactor Variation and Enzymatic Activity in Ogataea methanolica.Microbial biotechnology · 2026Article
- Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases.Synthetic and systems biotechnology · 2025Article
- Industrial applicability of enzymatic and whole-cell processes for the utilization of C1 building blocks.Nature communications · 2025Review
- Sustainable Bioconversion of Methanol: A Renewable Employing Novel Alcohol Oxidase and Pyruvate Aldolase.Journal of agricultural and food chemistry · 2025Article
- MftG is crucial for ethanol metabolism of mycobacteria by linking mycofactocin oxidation to respiration.eLife · 2025Article
- Novel Basidiomycetous Alcohol Oxidase fromInternational journal of molecular sciences · 2024Article
- Structure-Guided Evolution Modulate Alcohol Oxidase to Improve Ethanol Oxidation Performance.Applied biochemistry and biotechnology · 2024Article
- Glucose Oxidase, an Enzyme "Ferrari": Its Structure, Function, Production and Properties in the Light of Various Industrial and Biotechnological Applications.Biomolecules · 2022Review
- Crystallographic fragment screening-based study of a novel FAD-dependent oxidoreductase from Chaetomium thermophilum.Acta crystallographica. Section D, Structural biology · 2021Article
- Functional Classification of Super-Large Families of Enzymes Based on Substrate Binding Pocket Residues for Biocatalysis and Enzyme Engineering Applications.Frontiers in bioengineering and biotechnology · 2021Article
- Article
- The GMC superfamily of oxidoreductases revisited: analysis and evolution of fungal GMC oxidoreductases.Biotechnology for biofuels · 2019Article
- Oxidoreductases and Reactive Oxygen Species in Conversion of Lignocellulosic Biomass.Microbiology and molecular biology reviews : MMBR · 2018Review
- Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.Biochemistry · 2018Article
- Multiplicity of enzymatic functions in the CAZy AA3 family.Applied microbiology and biotechnology · 2018Review
- Genome scale identification, structural analysis, and classification of periplasmic binding proteins from Mycobacterium tuberculosis.Current genetics · 2017Article
- Implications of evolutionary engineering for growth and recombinant protein production in methanol-based growth media in the yeast Pichia pastoris.Microbial cell factories · 2017Article
- An extended N-H bond, driven by a conserved second-order interaction, orients the flavin N5 orbital in cholesterol oxidase.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
FAD-dependent alcohol oxidases (AOX) are key enzymes of methylotrophic organisms that can utilize lower primary alcohols as sole source of carbon and energy. Here we report the crystal structure analysis of the methanol oxidase AOX1 from Pichia pastoris. The crystallographic phase problem was solved by means of Molecular Replacement in combination with initial structure rebuilding using Rosetta model completion and relaxation against an averaged electron density map. The subunit arrangement of the homo-octameric AOX1 differs from that of octameric vanillyl alcohol oxidase and other dimeric or tetrameric alcohol oxidases, due to the insertion of two large protruding loop regions and an additional C-terminal extension in AOX1. In comparison to other alcohol oxidases, the active site cavity of AOX1 is significantly reduced in size, which could explain the observed preference for methanol as substrate. All AOX1 subunits of the structure reported here harbor a modified flavin adenine dinucleotide, which contains an arabityl chain instead of a ribityl chain attached to the isoalloxazine ring.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.