Evidence map›Paper›PMID 26905881›Full record

ArticleJournal of chromatography. A2016

Characterization of cross-linked cellulosic ion-exchange adsorbents: 2. Protein sorption and transport.

James M Angelo, Aleksandar Cvetkovic, Rene Gantier, Abraham M Lenhoff

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of chromatography. A, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 19 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

James M AngeloDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA.
Aleksandar CvetkovicPall Life Sciences, 20 Walkup Drive, Westborough, MA 01581, USA.
Rene GantierPall Life Sciences, 20 Walkup Drive, Westborough, MA 01581, USA.
Abraham M LenhoffDepartment of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA. Electronic address: lenhoff@udel.edu.
Pall Corporation (United States) · USUniversity of Delaware · US

Funding

Predictive Modeling & Optimal Control Framework for Model-Based Epidemic Response in DelawareP20GM103446 · NIGMS · UNIVERSITY OF DELAWARE · PI Anjana Narayan Bhat · 2012 to 2026
$67.2M
COBRE on Membrane Protein Production and CharacterizationP30GM103519 · NIGMS · UNIVERSITY OF DELAWARE · PI LENHOFF, ABRAHAM M · 2012 to 2014
$3.3M
NIGMS NIH HHS P20 GM103446NIGMS NIH HHS P30 GM103519
6 · The paper itself

Abstract

Adsorption behavior in the HyperCel family of cellulosic ion-exchange materials (Pall Corporation) was characterized using methods to assess, quantitatively and qualitatively, the dynamics of protein uptake as well as static adsorption as a function of ionic strength and protein concentration using several model proteins. The three exchangers studied all presented relatively high adsorptive capacities under low ionic strength conditions, comparable to commercially available resins containing polymer functionalization aimed at increasing that particular characteristic. The strong cation- and anion-exchange moieties showed higher sensitivity to increasing salt concentrations, but protein affinity on the salt-tolerant STAR AX HyperCel exchanger remained strong at ionic strengths normally used in downstream processing to elute material fully during ion-exchange chromatography. Very high uptake rates were observed in both batch kinetics experiments and time-series confocal laser scanning microscopy, suggesting low intraparticle transport resistances relative to external film resistance, even at higher bulk protein concentrations where the opposite is typically observed. Electron microscopy imaging of protein adsorbed phases provided additional insight into particle structure that could not be resolved in previous work on the bare resins.

Indexed as

AdsorptionChromatography, Ion ExchangeIon Exchange ResinsKineticsMicroscopy, ConfocalOsmolar ConcentrationProteinsIon Exchange ResinsProteinsCellulosic materialsConfocal laser scanning microscopyElectron microscopyIon-exchange chromatographyProtein adsorptionSalt-tolerant adsorbents

Identifiers

PMID26905881
PMCPMC4790824
OpenAlexW2266705390

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.