ArticleBMC infectious diseases2016
Validation of western blot for Histoplasma capsulatum antibody detection assay.
Article in BMC infectious diseases, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 41 citations in OpenAlex.
- Label-Free Profiling of Immunoglobulin Isotypes Using Solid-State Nanopores.Small methods · 2026Article
- Radiological features of chronic pulmonary histoplasmosis: Easily mistaken for tuberculosis.PLoS neglected tropical diseases · 2025Review
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- Multi-epitope protein production and its application in the diagnosis of opisthorchiasis.Parasites & vectors · 2024Article
- Histoplasmosis at a Reference Center for Infectious Diseases in Southeast Brazil: Comparison between HIV-Positive and HIV-Negative Individuals.Tropical medicine and infectious disease · 2023Article
- Immunologic Diagnosis of Endemic Mycoses.Journal of fungi (Basel, Switzerland) · 2022Review
- Pulmonary Histoplasmosis.Mycopathologia · 2021Review
- Acute Pulmonary Histoplasmosis Following COVID-19: Novel Laboratorial Methods Aiding Diagnosis.Journal of fungi (Basel, Switzerland) · 2021Article
- Chronic Pulmonary Histoplasmosis-A Scoping Literature Review.Open forum infectious diseases · 2020Article
- Immunoproteomics Reveals Pathogen's Antigens Involved inFrontiers in cellular and infection microbiology · 2020Article
- Association betweenOncology letters · 2018Article
- Costus root granules improve ulcerative colitis through regulation of TGF-β mediation of the PI3K/AKT signaling pathway.Experimental and therapeutic medicine · 2018Article
- Human fibroblast growth factor-21 serves as a predictor and prognostic factor in patients with hepatitis B cirrhosis combined with adrenal insufficiency.Experimental and therapeutic medicine · 2018Article
- Comparison of diagnostic methods to detect Histoplasma capsulatum in serum and blood samples from AIDS patients.PloS one · 2018Article
- Histoplasmosis in Africa: An emerging or a neglected disease?PLoS neglected tropical diseases · 2018Review
- Pulmonary histoplasmoma: a disguised malady.Autopsy & case reportsArticle
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHistoplasmosis is worldwide systemic mycoses caused by the dimorphic fungus Histoplasma capsulatum. The isolation and identification of H. capsulatum in culture is the reference test for histoplasmosis diagnosis confirmation. However, in the absence of it, serology has been used as a presumptive diagnosis through antibody and antigen detection. The purpose of the present study was to validate an immunoassay method (western blot) for antibodies detection in the diagnosis of histoplasmosis.
methodsTo validate the western blot (WB) a study was conducted using 118 serum samples from patients with histoplasmosis and 118 serum controls collected from January 2000 to December 2013 in residents of the Rio de Janeiro State, Brazil. Diagnostic validation parameters were calculated based on the categorization of results obtained in a 2 × 2 table and subjected to statistical analysis. In addition, the viability of deglycosylated histoplasmin antigen (ptHMIN) onto nitrocellulose membranes previously sensitized was evaluated during the same period.
resultsThe WB test showed sensitivity of 94.9 %, specificity of 94.1 %, positive predictive value of 94.1 %, negative predictive value of 94.9 %, accuracy of 94.5 %, and almost perfect precision. Besides, the strips have proved to be viable for using at least 5 years after ptHMIN antigen sensitization.
conclusionWestern blot test using ptHMIN provides sensitive, specific, and faster results. Therefore, could be considered a useful tool in the diagnosis of histoplasmosis being used by public health system, even in situations where laboratory facilities are relatively limited.
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