ArticleScience signaling2016
Dynamic regulation of neutrophil polarity and migration by the heterotrimeric G protein subunits Gαi-GTP and Gβγ.
Article in Science signaling, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Defining the human lung pathodegradome of the V8 protease fromInfection and immunity · 2026Article
- Nurr1 Orchestrates Claustrum Development and Functionality.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Germline mutations in a G protein identify signaling cross-talk in T cells.Science (New York, N.Y.) · 2024Article
- Actuation of single downstream nodes in growth factor network steers immune cell migration.Developmental cell · 2023Article
- Computational design of dynamic receptor-peptide signaling complexes applied to chemotaxis.Nature communications · 2023Article
- ELMO1 Deficiency Reduces Neutrophil Chemotaxis in Murine Peritonitis.International journal of molecular sciences · 2023Article
- Article
- Panel of human cell lines with human/mouse artificial chromosomes.Scientific reports · 2022Article
- A network of GαScience signaling · 2022Article
- Gαi protein subunit: A step toward understanding its non-canonical mechanisms.Frontiers in cell and developmental biology · 2022Review
- GαJournal of immunology (Baltimore, Md. : 1950) · 2021Article
- Effects of venoms on neutrophil respiratory burst: a major inflammatory function.The journal of venomous animals and toxins including tropical diseases · 2021Review
- Role and Regulation of Mechanotransductive HIF-1α Stabilisation in Periodontal Ligament Fibroblasts.International journal of molecular sciences · 2020Article
- Gβγ is a direct regulator of endogenous p101/p110γ and p84/p110γ PI3Kγ complexes in mouse neutrophils.Science signaling · 2020Article
- Insights into the Regulatory Properties of Human Adenylyl Cyclase Type 9.Molecular pharmacology · 2019Article
- Gβγ signaling to the chemotactic effector P-REX1 and mammalian cell migration is directly regulated by GαThe Journal of biological chemistry · 2019Article
- Targeting G protein-coupled receptor signalling by blocking G proteins.Nature reviews. Drug discovery · 2018Review
- Regulator of G protein signaling 5 restricts neutrophil chemotaxis and trafficking.The Journal of biological chemistry · 2018Article
- Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode.Developmental cell · 2018Article
- Activated heterotrimeric G protein αThe Journal of biological chemistry · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Activation of the Gi family of heterotrimeric guanine nucleotide-binding proteins (G proteins) releases βγ subunits, which are the major transducers of chemotactic G protein-coupled receptor (GPCR)-dependent cell migration. The small molecule 12155 binds directly to Gβγ and activates Gβγ signaling without activating the Gαi subunit in the Gi heterotrimer. We used 12155 to examine the relative roles of Gαi and Gβγ activation in the migration of neutrophils on surfaces coated with the integrin ligand intercellular adhesion molecule-1 (ICAM-1). We found that 12155 suppressed basal migration by inhibiting the polarization of neutrophils and increasing their adhesion to ICAM-1-coated surfaces. GPCR-independent activation of endogenous Gαi and Gβγ with the mastoparan analog Mas7 resulted in normal migration. Furthermore, 12155-treated cells expressing a constitutively active form of Gαi1 became polarized and migrated. The extent and duration of signaling by the second messenger cyclic adenosine monophosphate (cAMP) were enhanced by 12155. Inhibiting the activity of cAMP-dependent protein kinase (PKA) restored the polarity of 12155-treated cells but did not decrease their adhesion to ICAM-1 and failed to restore migration. Together, these data provide evidence for a direct role of activated Gαi in promoting cell polarization through a cAMP-dependent mechanism and in inhibiting adhesion through a cAMP-independent mechanism.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.