Evidence map›Paper›PMID 26904936›Full record

ArticleCell reports2016

NRF2 Orchestrates the Metabolic Shift during Induced Pluripotent Stem Cell Reprogramming.

Kate E Hawkins, Shona Joy, Juliette M K M Delhove, Vassilios N Kotiadis, Emilio Fernandez, Lorna M Fitzpatrick, James R Whiteford, Peter J King, Juan P Bolanos, Michael R Duchen and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 88 papers.

0numbers the graph read from it
0cells of the map it votes in
88citing papers in PubMed
10.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

88 citing papers in PubMed, 155 citations in OpenAlex.

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  9. The transcription factor BMI1 increases hypoxic signaling in oral cavity epithelia.Biochimica et biophysica acta. Molecular basis of disease · 2024
    Article
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  17. Metabolic control of induced pluripotency.Frontiers in cell and developmental biology · 2023
    Review
  18. Article
  19. Article
  20. Article

28 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 3 countries.

Kate E HawkinsStem Cell Group, Cardiovascular and Cell Sciences Research Institute, St. George's University of London, Cranmer Terrace, London SW17 0RE, UK. Electronic address: rmjbkeh@ucl.ac.uk.
Shona JoyStem Cell Group, Cardiovascular and Cell Sciences Research Institute, St. George's University of London, Cranmer Terrace, London SW17 0RE, UK; Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Juliette M K M DelhoveStem Cell Group, Cardiovascular and Cell Sciences Research Institute, St. George's University of London, Cranmer Terrace, London SW17 0RE, UK; Wits/SAMRC Antiviral Gene Therapy Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2000, South Africa; Gene Transfer Technology Group, Institute for Women's Health, University College London, 86-96 Chenies Mews, London WC1E 6HX, UK.
Vassilios N KotiadisDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Emilio FernandezInstitute of Functional Biology and Genomics, University of Salamanca-CSIC, 37007 Salamanca, Spain; Institute of Biomedical Research of Salamanca, University Hospital of Salamanca, 37007 Salamanca, Spain.
Lorna M FitzpatrickStem Cell Group, Cardiovascular and Cell Sciences Research Institute, St. George's University of London, Cranmer Terrace, London SW17 0RE, UK; School of Healthcare Science, John Dalton Building, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK.
James R WhitefordWilliam Harvey Research Institute, Charterhouse Square, Queen Mary University of London, London EC1M 6BQ, UK.
Peter J KingWilliam Harvey Research Institute, Charterhouse Square, Queen Mary University of London, London EC1M 6BQ, UK.
Juan P BolanosInstitute of Functional Biology and Genomics, University of Salamanca-CSIC, 37007 Salamanca, Spain; Institute of Biomedical Research of Salamanca, University Hospital of Salamanca, 37007 Salamanca, Spain.
Michael R DuchenDepartment of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK.
Simon N WaddingtonWits/SAMRC Antiviral Gene Therapy Research Unit, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg 2000, South Africa; Gene Transfer Technology Group, Institute for Women's Health, University College London, 86-96 Chenies Mews, London WC1E 6HX, UK.
Tristan R McKayStem Cell Group, Cardiovascular and Cell Sciences Research Institute, St. George's University of London, Cranmer Terrace, London SW17 0RE, UK; School of Healthcare Science, John Dalton Building, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, UK. Electronic address: t.mckay@mmu.ac.uk.
University College London · GBManchester Metropolitan University · GBSt George's, University of London · GBUniversidad de Salamanca · ESWilliam Harvey Research Institute · GB

Funding

INFLUENCE OF CANNABINOIDS ON ASTROCYTE-NEURONAL METABOLIC INTERACTIONSR21DA037678 · NIDA · UNIVERSITY OF SALAMANCA · PI BOLANOS, JUAN PEDRO · 2014 to 2015
$195k
Biotechnology and Biological Sciences Research Council BB/L020874/1European Research Council 260862Medical Research Council MC_PC_14118National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/L001780/1NIDA NIH HHS 1R21DA037678-01NIDA NIH HHS R21 DA037678Versus Arthritis 19207
6 · The paper itself

Abstract

The potential of induced pluripotent stem cells (iPSCs) in disease modeling and regenerative medicine is vast, but current methodologies remain inefficient. Understanding the cellular mechanisms underlying iPSC reprogramming, such as the metabolic shift from oxidative to glycolytic energy production, is key to improving its efficiency. We have developed a lentiviral reporter system to assay longitudinal changes in cell signaling and transcription factor activity in living cells throughout iPSC reprogramming of human dermal fibroblasts. We reveal early NF-κB, AP-1, and NRF2 transcription factor activation prior to a temporal peak in hypoxia inducible factor α (HIFα) activity. Mechanistically, we show that an early burst in oxidative phosphorylation and elevated reactive oxygen species generation mediates increased NRF2 activity, which in turn initiates the HIFα-mediated glycolytic shift and may modulate glucose redistribution to the pentose phosphate pathway. Critically, inhibition of NRF2 by KEAP1 overexpression compromises metabolic reprogramming and results in reduced efficiency of iPSC colony formation.

Indexed as

Cellular ReprogrammingDermisFibroblastsGene Expression RegulationGenes, ReporterGenetic VectorsGlycolysisHumansHypoxia-Inducible Factor 1, alpha SubunitInduced Pluripotent Stem CellsKelch-Like ECH-Associated Protein 1LentivirusLuciferasesNF-E2-Related Factor 2NF-kappa BOxidative PhosphorylationHIF1A protein, humanHypoxia-Inducible Factor 1, alpha SubunitKEAP1 protein, humanKelch-Like ECH-Associated Protein 1LuciferasesNFE2L2 protein, humanNF-E2-Related Factor 2NF-kappa BReactive Oxygen SpeciesTranscription Factor AP-1

Identifiers

PMID26904936
PMCPMC4785773
OpenAlexW2276858159

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.