ArticleInternational journal of bacteriology2013
Rapid PCR Detection of Mycoplasma hominis, Ureaplasma urealyticum, and Ureaplasma parvum.
Article in International journal of bacteriology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Hyperammonemia After Lung Transplantation: Systematic Review and a Mini Case Series.Transplant international : official journal of the European Society for Organ Transplantation · 2022Pooled it
- Evaluation of risk factors and treatment options associated with extra-urogenitalFrontiers in medicine · 2026Article
- Non-genitourinary Ureaplasma urealyticum infections in solid organ transplant recipients: a case report and literature review.BMC infectious diseases · 2025Review
- Proinflammatory Cytokines in Women with PCOS in Atypical Pathogen Infections.Diagnostics (Basel, Switzerland) · 2025Article
- Prevalence ofFrontiers in cellular and infection microbiology · 2025Article
- Chronic osteomyelitis with pathological fracture induced byFrontiers in medicine · 2025Article
- Donor-derived Mycoplasma and Ureaplasma infections in lung transplant recipients: A prospective study of donor and recipient respiratory tract screening and recipient outcomes.American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons · 2024Article
- Treatment of Hyperammonemia Syndrome in Lung Transplant Recipients.Journal of clinical medicine · 2023Review
- Routine Donor and Recipient Screening forOpen forum infectious diseases · 2022Article
- Flurofamide Prevention and Treatment ofMicrobiology spectrum · 2022Article
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- Contribution of Uremia toMicrobiology spectrum · 2022Article
- Development of a RPA-CRISPR-Cas12a Assay for Rapid, Simple, and Sensitive Detection ofFrontiers in microbiology · 2022Article
- Diagnostic parameters of the AF Genital System® for detection ofThe Journal of international medical research · 2021Observational
- Article
- Phenotypic Antimicrobial Susceptibility and Genotypic Characterization of ClinicalFrontiers in microbiology · 2021Article
- Septic arthritis of the shoulder due to Ureaplasma urealyticum after emergency caesarean section: a case report.BMC infectious diseases · 2020Article
- Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture.International journal of molecular sciences · 2020Article
- Hyperammonemia Post Lung Transplantation: A Review.Clinical medicine insights. Circulatory, respiratory and pulmonary medicine · 2020Review
- The Role ofClinical microbiology reviews · 2019Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objective. We compared laboratory developed real-time PCR assays for detection of Mycoplasma hominis and for detection and differentiation of Ureaplasma urealyticum and parvum to culture using genitourinary specimens submitted for M. hominis and Ureaplasma culture. Methods. 283 genitourinary specimens received in the clinical bacteriology laboratory for M. hominis and Ureaplasma species culture were evaluated. Nucleic acids were extracted using the Total Nucleic Acid Kit on the MagNA Pure 2.0. 5 μL of the extracts were combined with 15 μL of each of the two master mixes. Assays were performed on the LightCycler 480 II system. Culture was performed using routine methods. Results. M. hominis PCR detected 38/42 M. hominis culture-positive specimens, as well as 2 that were culture negative (sensitivity, 90.5%; specificity, 99.2%). Ureaplasma PCR detected 139/144 Ureaplasma culture-positive specimens, as well as 9 that were culture negative (sensitivity, 96.5%; specificity, 93.6%). Of the specimens that tested positive for Ureaplasma species, U. urealyticum alone was detected in 33, U. parvum alone in 109, and both in 6. Conclusion. The described PCR assays are rapid alternatives to culture for detection of M. hominis and Ureaplasma species, and, unlike culture, the Ureaplasma assay easily distinguishes U. urealyticum from parvum.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.