ArticleFrontiers in cellular and infection microbiology2016
The Two-Component System CpxRA Negatively Regulates the Locus of Enterocyte Effacement of Enterohemorrhagic Escherichia coli Involving σ(32) and Lon protease.
Article in Frontiers in cellular and infection microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Dynamic response by commensal E. coli two-component system Cpx contributes to C. elegans development.BMC biology · 2026Article
- Antibiotic-induced stress responses in Gram-negative bacteria and their role in antibiotic resistance.The Journal of antimicrobial chemotherapy · 2025Review
- A 5-HT-mediated urethral defense against urinary tract infections.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Virulence of Shigatoxigenic and EnteropathogenicVeterinary sciences · 2023Article
- Lepidopteran insects: emerging model organisms to study infection by enteropathogens.Folia microbiologica · 2023Review
- Bacterial virulence regulation through soluble peptidoglycan fragments sensing and response: knowledge gaps and therapeutic potential.FEMS microbiology reviews · 2023Article
- The Two-Component System CpxRA Represses Salmonella Pathogenicity Island 2 by Directly Acting on theMicrobiology spectrum · 2022Article
- Simulated Colonic Fluid Replicates theInfection and immunity · 2022Article
- cAMP Receptor Protein Positively Regulates the Expression of Genes Involved in the Biosynthesis ofFrontiers in microbiology · 2021Article
- Transcriptomic Analysis of Shiga Toxin-ProducingMicroorganisms · 2020Article
- The Serotonin Neurotransmitter Modulates Virulence of Enteric Pathogens.Cell host & microbe · 2020Article
- Taming the Beast: Interplay between Gut Small Molecules and Enteric Pathogens.Infection and immunity · 2019Review
- Article
- The CpxR regulates type VI secretion system 2 expression and facilitates the interbacterial competition activity and virulence of avian pathogenic Escherichia coli.Veterinary research · 2019Article
- Control freaks-signals and cues governing the regulation of virulence in attaching and effacing pathogens.Biochemical Society transactions · 2019Review
- The Interaction ofFrontiers in cellular and infection microbiology · 2019Article
- Maintaining Integrity Under Stress: Envelope Stress Response Regulation of Pathogenesis in Gram-Negative Bacteria.Frontiers in cellular and infection microbiology · 2019Review
- The Coli Surface Antigen CS3 of EnterotoxigenicFrontiers in microbiology · 2019Article
- Enterohemorrhagic Escherichia coli outwits hosts through sensing small molecules.Current opinion in microbiology · 2018Review
- Regulation of the Locus of Enterocyte Effacement in Attaching and Effacing Pathogens.Journal of bacteriology · 2018Review
Corrections and comments
- Retraction · 2025-06-11Concerns/Issues about Image · Duplication of/in Image · Investigation by Journal/Publisher · Investigation by Third Party · · See also: https://pubpeer.com/publications/17AFA8BBF7DDB1C36D8862778C19FA
- Retracted
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Enterohemorrhagic Escherichia coli (EHEC) is a significant cause of serious human gastrointestinal disease worldwide. EHEC strains contain a pathogenicity island called the locus of enterocyte effacement (LEE), which encodes virulence factors responsible for damaging the gut mucosa. The Cpx envelope stress response of E. coli is controlled by a two-component system (TCS) consisting of a sensor histidine kinase (CpxA) and a cytoplasmic response regulator (CpxR). In this study, we investigated the role of CpxRA in the expression of LEE-encoded virulence factors of EHEC. We found that a mutation in cpxA significantly affected adherence of EHEC to human epithelial cells. Analysis of this mutant revealed the presence of high levels of CpxR which repressed transcription of grlA and ler, the main positive virulence regulators of the LEE, and influenced negatively the production of the type 3 secretion system-associated EspABD translocator proteins. It is known that CpxR activates rpoH (Sigma factor 32), which in turns activates transcription of the lon protease gene. We found that transcription levels of ler and grlA were significantly increased in the lon and cpxA lon mutants suggesting that lon is involved in down-regulating LEE genes. In addition, the Galleria mellonella model of infection was used to analyze the effect of the loss of the cpx and lon genes in EHEC's ability to kill the larvae. We found that the cpxA mutant was significantly deficient at killing the larvae however, the cpxA lon mutant which overexpresses LEE genes in vitro, was unable to kill the larvae, suggesting that virulence in the G. mellonella model is T3SS independent and that CpxA modulates virulence through a yet unknown EHEC-specific factor. Our data provides new insights and broadens our scope into the complex regulatory network of the LEE in which the CpxA sensor kinase plays an important role in a cascade involving both global and virulence regulators.
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