ArticleFrontiers in plant science2016
Selection of Reference Genes for Normalization of MicroRNA Expression by RT-qPCR in Sugarcane Buds under Cold Stress.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Integrated transcriptomic and metabolomic profiling reveals coordinated regulatory networks associated with mosaic disease resistance in sugarcane.Frontiers in plant science · 2026Article
- Comprehensive Profiling of the miRNome and Degradome Reveals Regulatory Signatures of Seed Aging and Germination.International journal of molecular sciences · 2025Article
- Sugarcane microRNA shy-miR164 regulates sugar metabolism through direct cleavage of the transcription factor ScNAC mRNA.Plant physiology · 2025Article
- Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker.Scientific reports · 2024Article
- Identification of Low-Nitrogen-Related miRNAs and Their Target Genes in Sugarcane and the Role ofInternational journal of molecular sciences · 2022Article
- Integration of mRNA and microRNA analysis reveals the molecular mechanisms underlying drought stress tolerance in maize (Frontiers in plant science · 2022Article
- Selection and Optimization of Reference Genes for MicroRNA Expression Normalization by qRT-PCR in Chinese Cedar (International journal of molecular sciences · 2021Article
- Identification and validation of miRNA reference genes in poplar under pathogen stress.Molecular biology reports · 2021Article
- Selection of reference genes for normalization of microRNA expression in sugarcane stalks during its interaction with3 Biotech · 2021Article
- Article
- Enhanced sugar accumulation and regulated plant hormone signalling genes contribute to cold tolerance in hypoploid Saccharum spontaneum.BMC genomics · 2020Article
- Identification of Appropriate Reference Genes for Normalizing miRNA Expression in Citrus Infected byGenes · 2019Article
- Integrated mRNA and small RNA sequencing reveals microRNA regulatory network associated with internode elongation in sugarcane (Saccharum officinarum L.).BMC genomics · 2019Article
- RNA-seq-based selection of reference genes for RT-qPCR analysis of pitaya.FEBS open bio · 2019Article
- OsmiR528 Enhances Cold Stress Tolerance by Repressing Expression of Stress Response-related Transcription Factor Genes in Plant Cells.Current genomics · 2019Article
- Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar.Plant methods · 2019Article
- Selection of reference genes for miRNA qRT-PCR under abiotic stress in grapevine.Scientific reports · 2018Article
- Identifying optimal reference genes for the normalization of microRNA expression in cucumber under viral stress.PloS one · 2018Article
- Identification of Reference Genes for Analysis of microRNA Expression Patterns in Equine Chorioallantoic Membrane and Serum.Molecular biotechnology · 2018Article
- miRNA alteration is an important mechanism in sugarcane response to low-temperature environment.BMC genomics · 2017Article
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9 authors.
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Abstract
Sugarcane, accounting for 80% of world's sugar, originates in the tropics but is cultivated mainly in the subtropics. Therefore, chilling injury frequently occurs and results in serious losses. Recent studies in various plant species have established microRNAs as key elements in the post-transcriptional regulation of response to biotic and abiotic stresses including cold stress. Though, its accuracy is largely influenced by the use of reference gene for normalization, quantitative PCR is undoubtedly a popular method used for identification of microRNAs. For identifying the most suitable reference genes for normalizing miRNAs expression in sugarcane under cold stress, 13 candidates among 17 were investigated using four algorithms: geNorm, NormFinder, deltaCt, and Bestkeeper, and four candidates were excluded because of unsatisfactory efficiency and specificity. Verification was carried out using cold-related genes miR319 and miR393 in cold-tolerant and sensitive cultivars. The results suggested that miR171/18S rRNA and miR171/miR5059 were the best reference gene sets for normalization for miRNA RT-qPCR, followed by the single miR171 and 18S rRNA. These results can aid research on miRNA responses during sugarcane stress, and the development of sugarcane tolerant to cold stress. This study is the first report concerning the reference gene selection of miRNA RT-qPCR in sugarcane.
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