Evidence map›Paper›PMID 26903627›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2016

Quantitative proteomic analyses of mammary organoids reveals distinct signatures after exposure to environmental chemicals.

Katherine E Williams, George A Lemieux, Maria E Hassis, Adam B Olshen, Susan J Fisher, Zena Werb

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
5.1field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.

  1. Pooled it
  2. Review
  3. Review
  4. Onco-Breastomics: An Eco-Evo-Devo Holistic Approach.International journal of molecular sciences · 2024
    Review
  5. Article
  6. Article
  7. Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. Article
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  15. Article
  16. Review
  17. Article
  18. Kidney organoids in translational medicine: Disease modeling and regenerative medicine.Developmental dynamics : an official publication of the American Association of Anatomists · 2020
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Katherine E WilliamsDepartment of Obstetrics and Gynecology, University of California, San Francisco, CA 94143;
George A LemieuxDepartment of Anatomy, University of California, San Francisco, CA 94143;
Maria E HassisDepartment of Obstetrics and Gynecology, University of California, San Francisco, CA 94143;
Adam B OlshenDepartment of Epidemiology and Biostatistics, University of California, San Francisco, CA 94143.
Susan J FisherDepartment of Obstetrics and Gynecology, University of California, San Francisco, CA 94143;
Zena WerbDepartment of Anatomy, University of California, San Francisco, CA 94143; zena.werb@ucsf.edu.
University of California, San Francisco · US

Funding

Environmental Effect on the Mammary Gland across the LifespanU01ES019458 · NIEHS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WERB, ZENA · 2010 to 2014
$2.5M
NIEHS NIH HHS ES019458NIEHS NIH HHS U01 ES019458
6 · The paper itself

Abstract

Common environmental contaminants such as bisphenols and phthalates and persistent contaminants such as polychlorinated biphenyls are thought to influence tissue homeostasis and carcinogenesis by acting as disrupters of endocrine function. In this study we investigated the direct effects of exposure to bisphenol A (BPA), mono-n-butyl phthalate (Pht), and polychlorinated biphenyl 153 (PCB153) on the proteome of primary organotypic cultures of the mouse mammary gland. At low-nanomolar doses each of these agents induced distinct effects on the proteomes of these cultures. Although BPA treatment produced effects that were similar to those induced by estradiol, there were some notable differences, including a reduction in the abundance of retinoblastoma-associated protein and increases in the Rho GTPases Ras-related C3 botulinum toxin substrate 1 (Rac1) and cell division cycle protein CDC42. Both Pht and PCB153 induced changes that were distinct from those induced by estrogen, including decreased levels of the transcriptional corepressor C-terminal binding protein 1. Interestingly, the three chemicals appeared to alter the abundance of distinct splice forms of many proteins as well as the abundance of several proteins that regulate RNA splicing. Our combined results indicate that the three classes of chemical have distinct effects on the proteome of normal mouse mammary cultures, some estrogen-like but most estrogen independent, that influence diverse biological processes including apoptosis, cell adhesion, and proliferation.

Indexed as

AnimalsBenzhydryl CompoundsBisphenol A CompoundsChromatography, High Pressure LiquidCluster AnalysisEnvironmental PollutantsEstrogens, Non-SteroidalFemaleHumansMammary Glands, AnimalMass SpectrometryMiceOrganoidsPhenolsPhthalic AcidsPolychlorinated BiphenylsBenzhydryl Compoundsbisphenol ABisphenol A CompoundsEnvironmental PollutantsEstrogens, Non-Steroidalmonobutyl phthalatePhenolsPhthalic AcidsPolychlorinated BiphenylsProteomeenvironmental chemicalsestrogenmammary epitheliumorganotypic cultureproteomics

Identifiers

PMID26903627
PMCPMC4790982
OpenAlexW2279768526

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.