ArticleThe Journal of biological chemistry2016
Molecular Insights into Toluene Sensing in the TodS/TodT Signal Transduction System.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Microbial and multi-omics approaches for bioremediation of emerging contaminants: environmental impact and future engineering solutions.Biodegradation · 2026Review
- Structural and functional diversity of sensor domains in bacterial transmembrane receptors.Trends in microbiology · 2025Review
- Thermal shift assay to identify ligands for bacterial sensor proteins.FEMS microbiology reviews · 2025Review
- Genetic Analysis Reveals a Requirement for the Hybrid Sensor Kinase RscS inJournal of bacteriology · 2023Article
- Article
- Fluoro-recognition: New in vivo fluorescent assay for toluene dioxygenase probing induction by and metabolism of polyfluorinated compounds.Environmental microbiology · 2022Article
- Antimicrobial resistance: progress and challenges in antibiotic discovery and anti-infective therapy.Microbial biotechnology · 2022Article
- Phylogenetic Analysis with Prediction of Cofactor or Ligand Binding for Pseudomonas aeruginosa PAS and Cache Domains.Microbiology spectrum · 2021Article
- Transcriptomic analysis of Burkholderia cenocepacia CEIB S5-2 during methyl parathion degradation.Environmental science and pollution research international · 2021Article
- PAS domains in bacterial signal transduction.Current opinion in microbiology · 2021Review
- Tetrameric architecture of an active phenol-bound form of the AAANature communications · 2020Article
- Benzylsuccinate Synthase is Post-Transcriptionally Regulated in the Toluene-Degrading DenitrifierMicroorganisms · 2020Article
- Crystal structure of theThe Journal of biological chemistry · 2019Article
- Transcriptional Regulation of the Peripheral Pathway for the Anaerobic Catabolism of Toluene andFrontiers in microbiology · 2018Article
- New family of biosensors for monitoring BTX in aquatic and edaphic environments.Microbial biotechnology · 2016Article
Corrections and comments
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
TodS is a sensor kinase that responds to various monoaromatic compounds, which either cause an agonistic or antagonistic effect on phosphorylation of its cognate response regulator TodT, and controls tod operon expression in Pseudomonas putida strains. We describe a molecular sensing mechanism of TodS that is activated in response to toluene. The crystal structures of the TodS Per-Arnt-Sim (PAS) 1 sensor domain (residues 43-164) and its complex with toluene (agonist) or 1,2,4-trimethylbenzene (antagonist) show a typical β2α3β3 PAS fold structure (residues 45-149), forming a hydrophobic ligand-binding site. A signal transfer region (residues 150-163) located immediately after the canonical PAS fold may be intrinsically flexible and disordered in both apo-PAS1 and antagonist-bound forms and dramatically adapt an α-helix upon toluene binding. This structural change in the signal transfer region is proposed to result in signal transmission to activate the TodS/TodT two-component signal transduction system. Site-directed mutagenesis and β-galactosidase assays using a P. putida reporter strain system verified the essential residues involved in ligand sensing and signal transfer and suggest that the Phe(46) residue acts as a ligand-specific switch.
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