ArticleBrain research2016
Retrograde optogenetic characterization of the pontospinal module of the locus coeruleus with a canine adenoviral vector.
Article in Brain research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 110 citations in OpenAlex.
- Circuit-selective pharmacological targeting of prefrontal cortex-projecting locus coeruleus neurons drives antinociception.Cell reports · 2025Article
- The Locus Coeruleus-Noradrenergic System in the Healthy and Diseased Brain: A Narrative Review.European journal of neurology · 2025Review
- A comparison of viral strategies and model systems to target norepinephrine neurons in the locus coeruleus reveals high variability in transgene expression patterns.PLoS biology · 2025Article
- Article
- Aging disrupts locus coeruleus-driven norepinephrine transmission in the prefrontal cortex: Implications for cognitive and motor decline.Aging cell · 2025Article
- Variations of neuronal properties in the region of locus coeruleus of mice.Brain research · 2024Article
- Diversity of ancestral brainstem noradrenergic neurons across species and multiple biological factors.bioRxiv : the preprint server for biology · 2024Article
- Multiple Posterior Insula Projections to the Brainstem Descending Pain Modulatory System.International journal of molecular sciences · 2024Article
- Periaqueductal gray activates antipredatory neural responses in the amygdala of foraging rats.eLife · 2024Article
- Inputs to the locus coeruleus from the periaqueductal gray and rostroventral medulla shape opioid-mediated descending pain modulation.Science advances · 2024Article
- Sex differences in single neuron function and proteomics profiles examined by patch-clamp and mass spectrometry in the locus coeruleus of the adult mouse.Acta physiologica (Oxford, England) · 2024Article
- Virus-Based Neural Circuit Tracing.Advances in neurobiology · 2024Review
- Shining light on the noradrenergic system.Neurophotonics · 2023Review
- Neuronal diversity of neuropeptide signaling, including galanin, in the mouse locus coeruleus.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- A critical brainstem relay for mediation of diffuse noxious inhibitory controls.Brain : a journal of neurology · 2023Article
- Tauopathy and alcohol consumption interact to alter locus coeruleus excitatory transmission and excitability in male and female mice.Alcohol (Fayetteville, N.Y.) · 2023Article
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- Article
- A noradrenergic-hypothalamic neural substrate for stress-induced sleep disturbances.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Brainstem noradrenergic neurons: Identifying a hub at the intersection of cognition, motility, and skeletal muscle regulation.Acta physiologica (Oxford, England) · 2022Review
1 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
Noradrenergic neurons of the brainstem extend projections throughout the neuraxis to modulate a wide range of processes including attention, arousal, autonomic control and sensory processing. A spinal projection from the locus coeruleus (LC) is thought to regulate nociceptive processing. To characterize and selectively manipulate the pontospinal noradrenergic neurons in rats, we implemented a retrograde targeting strategy using a canine adenoviral vector to express channelrhodopsin2 (CAV2-PRS-ChR2-mCherry). LC microinjection of CAV2-PRS-ChR2-mCherry produced selective, stable, transduction of noradrenergic neurons allowing reliable opto-activation in vitro. The ChR2-transduced LC neurons were opto-identifiable in vivo and functional control was demonstrated for >6 months by evoked sleep-wake transitions. Spinal injection of CAV2-PRS-ChR2-mCherry retrogradely transduced pontine noradrenergic neurons, predominantly in the LC but also in A5 and A7. A pontospinal LC (ps:LC) module was identifiable, with somata located more ventrally within the nucleus and with a discrete subset of projection targets. These ps:LC neurons had distinct electrophysiological properties with shorter action potentials and smaller afterhyperpolarizations compared to neurons located in the core of the LC. In vivo recordings of ps:LC neurons showed a lower spontaneous firing frequency than those in the core and they were all excited by noxious stimuli. Using this CAV2-based approach we have demonstrated the ability to retrogradely target, characterise and optogenetically manipulate a central noradrenergic circuit and show that the ps:LC module forms a discrete unit. This article is part of a Special Issue entitled SI: Noradrenergic System.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.