ArticleInvestigative ophthalmology & visual science2016
In Vivo Two-Photon Fluorescence Kinetics of Primate Rods and Cones.
Article in Investigative ophthalmology & visual science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 42 citations in OpenAlex.
- Reflective broadband adaptive optics scanning light ophthalmoscope with 20 D focus range.Biomedical optics express · 2026Article
- Brillouin microscopic assessment of retinal stiffness during reactive Müller gliosis.Experimental eye research · 2026Article
- [Emerging technology for retinal function testing: two-photon microperimetry].Die Ophthalmologie · 2026Review
- On the physiological processes underlying optoretinography [Invited].Biomedical optics express · 2025Review
- In Vivo Visualization of Intravascular Patrolling Immune Cells in the Primate Eye.Investigative ophthalmology & visual science · 2024Article
- Suppression of Subpixel Jitter in Resonant Scanning Systems With Phase-locked Sampling.IEEE transactions on medical imaging · 2024Article
- Two-photon autofluorescence lifetime assay of rabbit photoreceptors and retinal pigment epithelium during light-dark visual cycles in rabbit retina.Biomedical optics express · 2024Article
- Lipofuscin, Its Origin, Properties, and Contribution to Retinal Fluorescence as a Potential Biomarker of Oxidative Damage to the Retina.Antioxidants (Basel, Switzerland) · 2023Review
- Evolution of adaptive optics retinal imaging [Invited].Biomedical optics express · 2023Review
- Separate lifetime signatures of macaque S cones, M/L cones, and rods observed with adaptive optics fluorescence lifetime ophthalmoscopy.Scientific reports · 2023Article
- Human cone elongation responses can be explained by photoactivated cone opsin and membrane swelling and osmotic response to phosphate produced by RGS9-catalyzed GTPase.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Optogenetic therapy restores retinal activity in primate for at least a year following photoreceptor ablation.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- In vivo imaging of the human eye using a 2-photon-excited fluorescence scanning laser ophthalmoscope.The Journal of clinical investigation · 2022Article
- Adaptive optics two-photon excited fluorescence lifetime imaging ophthalmoscopy of photoreceptors and retinal pigment epithelium in the living non-human primate eye.Biomedical optics express · 2022Article
- Report From the National Eye Institute Workshop on Neuro-Ophthalmic Disease Clinical Trial Endpoints: Optic Neuropathies.Investigative ophthalmology & visual science · 2021Article
- Adaptive optics: principles and applications in ophthalmology.Eye (London, England) · 2021Review
- Imaging Transplanted Photoreceptors in Living Nonhuman Primates with Single-Cell Resolution.Stem cell reports · 2020Article
- Localized Photoreceptor Ablation Using Femtosecond Pulses Focused With Adaptive Optics.Translational vision science & technology · 2020Article
- Organ Cultures for Retinal Diseases.Frontiers in neuroscience · 2020Review
- Adaptive optics two-photon microscopy enables near-diffraction-limited and functional retinal imaging in vivo.Light, science & applications · 2020Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
purposeThe retinoid cycle maintains vision by regenerating bleached visual pigment through metabolic events, the kinetics of which have been difficult to characterize in vivo. Two-photon fluorescence excitation has been used previously to track autofluorescence directly from retinoids and pyridines in the visual cycle in mouse and frog retinas, but the mechanisms of the retinoid cycle are not well understood in primates.
methodsWe developed a two-photon fluorescence adaptive optics scanning light ophthalmoscope dedicated to in vivo imaging in anesthetized macaques. Using pulsed light at 730 nm, two-photon fluorescence was captured from rods and cones during light and dark adaptation through the eye's pupil.
resultsThe fluorescence from rods and cones increased with light exposure but at different rates. During dark adaptation, autofluorescence declined, with cone autofluorescence decreasing approximately 4 times faster than from rods. Rates of autofluorescence decrease in rods and cones were approximately 4 times faster than their respective rates of photopigment regeneration. Also, subsets of sparsely distributed cones were less fluorescent than their neighbors immediately following bleach at 565 nm and they were comparable with the S cone mosaic in density and distribution.
conclusionsAlthough other molecules could be contributing, we posit that these fluorescence changes are mediated by products of the retinoid cycle. In vivo two-photon ophthalmoscopy provides a way to monitor noninvasively stages of the retinoid cycle that were previously inaccessible in the living primate eye. This can be used to assess objectively photoreceptor function in normal and diseased retinas.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.